XB-CLONE-4352494
Clone Name: IMAGE:7027418
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Gene:
tbl2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784214
[+]
taattaatcccccccccccccggtgacgtggcccagcgcttgttggacatggaggcggccgtggcagtggttgtggtgtcggtgctgttgggaggaatta
tcctgcttctggccttactgggaaagcgcccacagagcgacagcgaacaggacctgagcgccaaaaaagcaaatggagaagctgcaccagagaaactacc
ggcaaagaagccaaaactgcagaagctgcgcaaagaaaagccccagcaacacaattttacacaccggctccttgcagcctctctcaaggcacacagtggg
aatgtgacatgcttagatttcagcagtaatggtaaataccttgcatcctgctctgatgacagaacagtgaggatatggagcaccaaggattttctagatc
gagaacaccgcagcatgagggctaatgtagagttggaccatgctacacatgttcgatttagcccagactgtcgggcatttatagttttcttggctaatgg
tgagaccattcgggtctttaaaatgaccaaaaaggaagatggcttcaccttcacgccggctcctgaagactttgccaagagacctaaagccccccttatt
aacattggaaatacacaaacttgggaagtttatcatgaaccgctttcaatgaaaaccactattttaatttggggaatttaaaggggcgaaggtactgggc
cttcaattttttacccaacccaaaatgaaaaanattgccataaaccttgcctaatcatctccctttggggggaaaaattttgtaggggcaaacctcgttg
ggctttcccacctccccagaaattcccaaaacctcttttcgggaaagggttggtgccctttttggc
3' EST: CF784213
[+]tttttttttttttttctcacactgaattccacaggaaatttaatttatgcagaatacagaaatatctttgttaaaaaaaacaaaaaaaggtgttgaaggt
ccatcagtttgtctgcttgcacacagcatccagggcactctgtgcatcaatgatctgttgttgtagcctgtcttttgtgcctttattggtggctttcttt
agcatgtccttcatgtcaccaatggctgctctgtaccctgccgtgttgtggaacacacgtatagcgcgatctccagtggagaccaggaatctattgttgg
tatcaaaaaggaggtccgtaatgacatccccatgcaccgaatggaactcctcctcaactgcccctgttcgtgtgttgaacactgtaatgtcggaaatgct
ggaaatggccagaacacgagcatcgggggacagagctatccggcagggattgcttacagagatctgccctgtgtacagcaggtacgggtcttgctgcttt
ttgtattcaacatctgtatcccacagcttccaagtcccatccttagatacagtagccatcctgcgggagtcattggagaaagcaaatccatacacaccgg
cagaatgccccttcagatcaaatgctctagacacctcacggaattctccttgctttccaaagcacacctcccaaacttttacatctggagtgaaaccaca
gcttgccacaaatctcccacaaggagagatagcagcatatgcatttattcatcgggttggttaatgaatttgagcccagtaccttcccccttttaaatcc
caaatttaaaataaggggtgtccatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: