XB-CLONE-4352489
Clone Name: IMAGE:7027416
|
Gene:
gclm
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784217
[+]
taattaatccccccccccccccgaatgtttgcgagagctgcacatggttctctatccaccggtagcaggcaagcagcaggggagtgcgcaggtttccgta
agtcacggttacacagcacttttataacggggcctaatttctgctaagtcaccgtcctgtgcaaataagaccgcctggccttcacattcagagaggaaac
ggctttggacgcactttaactgcgacatggggacagacagcattgctgctcgcaccttgctggacaaggcggacaaactcgttatacagactggtaacct
gctaaactggggatgcctgaagaagaaatgctctagcacccccagcgaagagttacaagattgcatcagaacaacgttatgcgaatggagccaaaatttc
attccagaacttgaaaaggatatccctcccacacttgattgtaccagttccttcaggcaatggcaaaccataacttttttggtctaatgaataaacaaat
tggaaagctctccttgtgaattgtctcattggctgaggttcccagctcattttaattctagtcatctacccgggtccctttagccaattgggcgttcggg
tttaaaatacaccattgggtaaattttggcctcctttttattcagcatttttcttaaaatgggaatctcaatgttgggggttcccacaccccatttcgtt
aattttggtaaaaggaatatgggggtaaaacaaaacattttgttttatctttttttggaccagggaactttgttggccacagcccctcttatatagggag
agaaaaaacaaccccctttatataccgccacccctgttttttctgaaaacaattggcggacaaacatgtgtgggttttatcccacccttagaggtgataa
ctttttttttaccccttccctttatttactcccgccctttcttattctgccaaccccgttttttttacacaaaataagcggcgtctcccaaaacgtataa
aaaggtccaagcactcaactttaaatacgagaggcgtacaccactctattctaatttatttcccccaccaaattataggggatattattcgaaaaccctt
caga
3' EST: CF784216
[+]tttttttttttttttacctggttttagtgtgttttaattaatctccaaagttacaaaatgacctataaaacaatttactgattaccagatatatgtgact
caatacatcattctgtgttagaaaaacaaaatgtctatagcatattttcagtagcctccaacataaaacagcaattttatagactagatgttggcccagt
gtagtacatgttaaaacaatacacacttcctacaatagcagagcatgctctccatactacagattgcagttttgtcataaattagctcattttgtaaaca
aatctctgtatatatagataatacaattgaactgacaaacttggtcttgtaaataaatgcctccatttacgtaaaatagctgtgcgtctgagaaccaggt
cagtgtgctttattggggctaaagggaacctttcctttgagcctgcaaaatgtatccttttaatttaataattcctcgtgttttcactatgacagagtat
cttaaaatccagataggtgaccaggcactggaatgacattcttgtgcagattctttcagggcttcttgaaaggcttcttcagaaaggagctctttggggt
cattgtgtgttagcagctggatgtcaaactgttttgcaaactctgtaagatcaggtggcattatacagcatgatgcaaggttcacttgattactggctgg
cttttactgggaacagaggtaaagctgttccaaaagtgctttatccaagtctgatgtccctatggaaaccactttttcattttcaacaagactt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: