XB-CLONE-4352481
Clone Name: IMAGE:7027413
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784222 [+]
taattaatccccccccccccccgcctcttgtcattggtgccatcttaggaaggcctttcttctcgagatgagagccaagtggaggaagaagcgtatgcgc
aggctgaagcgcaaaaggagaaagatgagacaaaggtccaagtaaacttctcctccattgctgcctttgagactggcaagctgaacatagctctcttgaa
cagactatgaagttcatcaccaattagccatcgcaagggagctgtcctgcactagactgaaccctaaccacagtctgcaagcttttgccaagtttgccag
ggttgaaaggatctgcagttataaacagccactgaagatacttgcatcacctgctgtcaggagtttggacttgtttgctgtctcatgtagcaagtgtaat
taaagctgtctgttaaattccaaaacaaaacaaaa
3' EST: CF784221 [+]tttttttttttttttgaatttaacaaacagctttaattacacttgctacatgagacagcaaacaagtccaaactcctgacagcaggtgatgcaagtatct
tcagtggctgtttataactgcagatcctttcaaccctggcaaacttggcaaaagcttgcagactgtggttagggttcagtctagtgcaggacagctccct
tgcgatggctaattggtgatgaacttcatagtctgttcaagagagctatgttcagcttgccagtctcaaaggcagcaatggaggagaagtttacttggac
ctttgtctcatctttctccttttgcgcttcagcctgtgcatacgcttcttcctccacttggctctcatctcaaaaagaaaggccttcctaagatggcacc
aatgacaagaggcggggggggggggggattaattaactcgagtgcataaaaaaccagcaccttgtattctggccctgaatagaacctatgtccacctgcg
acctggaaagtgggacaccctggtggaaaagaaagcgccaaacgtggaattgtttatttcctatacagcggtcaccccaaaaatcacgaaaaagtggtca
tggaaacccaccggaagggtttattggtactataaatccgcgccccgcccctgcccctcttttataccggagacacctaaaaggcggtcggttaggggtc
tctatatataacaactctacccaaagagggggcggggtaacaaaatacacggtgccacggcccctcttaggcgaaacgggggtggaagtatttgacgatg
gtgccacacaaattacaaacacaacgtatccctacactagacagctcgatttagatcgctccggacattatgtggatcacccccgagcaggatccctnac
tccctgctgcatnaagcgggtaaatatataataaaccgtaggcggctgtcggcgctgatctcctacctcaatttcacactccccaccctccacgtccttt
tttttaataataatacaataaaacccccccccccctctctatacaacaccacgccccccgccgccataaaacaaaaaaaaaaaaaacccactcgtgcag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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