XB-CLONE-4352461
Clone Name: IMAGE:7027405
|
Gene:
snrpb2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC084519 [+]
AGCTTGGAGCCGTAAGGCGCACGGAAGCGTGTGTGAGCGTATTTAGAATATACAAGCCATGGATATTCGGCCAAATCACACTGTCTACATCAACAACCTC
TGTGATAAAGTAAAAAAACCGGAACTAAAGCGATCATTGTATGCGCTATTCTCCCAGTTTGGCCATGTTGTAGACATTGTGGCATTAAAGACCATGAAAA
TGAGAGGACAAGCGTTTGTAATCTTCAAAGAGCTGAGTTCTGCTACAAATGCTCTGCGGCAGTTGCAGGGTTTCCCTTTTTATAATAAACCAATGCGCAT
CCAGTATGCAAAGAGCGACTCTGATGTGATACTAAAGATGAAAGGTACATTTGCTGATAAAGAGAAGAAAAAAGAGAAGAAGAAAGCCAAAGCACAAGAA
CAGGCAGCTAATGCTGCAAATAAAAAGCCTGCTCTGGCATCAAATGCAAATAATGCACCAGGAGCATCACAGAATCAGCAGGTGCCGGACAACCCGCCAA
ATTACATCCTCTTTCTTAATAATTTGCCTGAGGAGACCAATGAGATGATGCTTTCTATGTTATTTAACCAATTCCCTGGCTTCAAAGAAGTGCGTTTGGT
CCCCGGGAGGCACGATATTGCGTTTGTGGAGTTTGAGAATGAAACGGAGGCAGGAGCTGCTCGAGATGCACTTCAGGGATTTAAGATCACACCATCTCAT
GCTATGAAAATTACCTACGCTAATAAATAGAGCAGAAGCGCGACACTGATGGACTTATATGTTTTTGTTCTGCCCCACTGCCCCCTATGAACTACTTTAA
GAAGTCCTTTTTATTGGTGAAGGAGAAAAGGCTGTTTTATTCCTGAACACCCTATTTTGTATGAATAAATGTTATTTTTTTGTCTACTAAGCAAATTTTG
CATTGCATTTCAGCCCTAAAAAAGCAAAGGGTTTTGGGTAATACAGAGATACAGAGTTGGTTCTGTTGTATTATAATCTGTTAAGTACTGTACACTGAAT
TAGCAGTGCATTTAATTCTTCCCTCGCAATAAAAGCAAAGTTGCATTTTTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF784234 [+]taattaatcccccccccccccagcttggagccgtaaggcgcacggaagcgtgtgtgagcgtatttagaatatacaagccatggatattcggccaaatcac
actgtctacatcaacaacctctgtgataaagtaaaaaaaccggaactaaagcgatcattgtatgcgctattctcccagtttggccatgttgtagacattg
tggcattaaagaccatgaaaatgagaggacaagcgtttgtaatcttcaaagagctgagttctgctacaaatgctctgcggcagttgcagggtttcccttt
ttataataaaccaatgcgcatccagtatgcaaagagcgactctgatgtgatactaaagatgaaaggtacatttgctgataaagagaagaaaaaagagaag
aagaaagccaaagcacaagaacaggcagctaatgctgcaaataaaaagcctgctctggcatcaaatgcaaataatgcaccaggagcatcacagaatcagc
aggtgccggacaacccgccaaattacatcctctttcttaataatttgcctgaggagaccaatgagatgatgctttctatgttatttaaccaattccctgg
cttcaaagaagtgcgtttggtccccgggaggcacgatattgcgtttgtggagtttgagaatgaaacggaggcaggagctgctcgagatgcacttcaggga
tttaagatcacaccatctcatgctatgaaaaatacctacgctaaataatagagcagaagccgcgacactgaagggaactaaatgttttttgtttcggccc
cactgcccccctaagga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: