XB-CLONE-4352455
Clone Name: IMAGE:7027403
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784238
[+]
taattaatcccccccccccccgagccgctctgttctgtatatacttcgatacaaaggccactagaaccctatacacagtgctgggaaagtaaggggcctg
agagagtgttcatgagaggtcgggggaacttcctgaactcattaaatgtcggctgtgtgagagcgcagggcaggtgtaaatgatcagagtgcagcccaca
aatacacactgagaggaatacaaagtataatgaatctgtgggagaatcgcttgccatacaatgtccggtcagtttctgctccaaagaactcccagttgtg
agttggtgcaagtttgtaggggaaacctgtgagtctctcagcgcggggccggggcggctcatggaatggacggaccggaaggaaaatacagccgtttact
tactaacgtttgtaggggcccaactgaatgacactgggtattatcgctgcactttaaccacccaatcagtgaacactgtgggccgtacagtgatggtcaa
aatcattggccataaggaaaggacgactgcccagctgcccagtacaacccatattatgggcggctgctcccgagttgaaggcagcactcagcctggttac
tggggtgatattgcttgaaaatcccccttgccaattgggggcccacatcaccttgtggttttattgtcttggggtacatataacccgtctaaccgaaata
tttttccaaaaaactctttcaaaagggcaaaaacgtttacacggtattctatttgcccccccaaaatatttcacccataataacttgccggttacgataa
ccttcccatcttgcgagacatttgggaattcttcccgggacaagatttctctgggcggctccgggtgtttttatcgggc
3' EST: CF784237
[+]tttttttttttttttgtaagtacagatacaaaagaagctaaatgggtcctttatccataagtcatataaacatttatttttctagctacaaaaatgtatt
ctatgcagtttggttttgttctttgtcgttggaatcttccacagagctgcatgtttcctccgccgtaatgttattataggtgatttcattggctgaggcc
gggcccggagtgtgcagagatccaatgtccaacggaggctctacagcacttattggtggcgttttggaggcattgatacctgtaacgttttcccttcgca
gagtctttgaaaatcttctgtttgcggttattgtacccaggcaataaaacagcagtgagctggccccaattactatgaggattctcagcaatatcaccca
gtagccgagcctggtgctgccttcaactccggagcagccgccccttgtatgggttgtactgggcagctgggcagtcgtcctttccttatggccagtgatt
ttgaccatcactgtacggcccacagtgttcactgactgggtggttaaagtgcagcgataatacccagtgtcattcagttgggcccctacaaacgttagta
agtaaacggctgtattttccttccggtccgtccattccatgagccgccccggccccgcgctgagagactcacaggtttcccctacaaacttgcaccaact
cacaactgggagttctttgggagcagaaaactgaccggacattgtatggccaagcgattctcccacagaatcaataaactttgtattcctctcaaggttg
gattttgggggctgcaactcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: