XB-CLONE-4352448
Clone Name: IMAGE:7027400
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784242 [+]
taattaatcccccccccccccaaatccttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
ttttttttttttttttttttttttttttttttttttttttttttttttttttttttccccccttttttttttttttttttttttttttttcccccccccc
aaaaaaatttttccccaaaaaaaaaaaaaaggggtttttttttttttggggggccccccccgggggggggggggggggggcccccctttttttttgggcc
ccttagggggggaaaaaaaaaaaaaaggcccccaattttttaaaaaaaaaatttttttttccccgccccatgggggggggggggccccccccccgggggt
tttttttggggggggggggggaaaaaaattgcggcccccccggggggggaaaaaacccctgaaaaaaagagggggggggggttctcttgggggggaaaaa
aaacccgggggggggggggggtctaggggggggagaaacccccccccccccccccgggggaaaaaaaaaacaaaccctcttttttttttacccaccccgg
gggggggggaggcccccccccccccccccgggggaaaaaaatgtgnacggctttcttttattttgtgggaaaaaatggggggcgcccacaaaaatatggc
gcctccccccgcacaaaaaaaagggaggagatggggcctattttcctcccccgggggggggggggaaaaaaatatttttttttttttatgggggagagag
agaggggggggggggcgccccccgccacataataaaggagaaaaatttaaaa
3' EST: CF784241 [+]ttttttttttttttggattaaatcttttttggagatgaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaagaaagaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaacaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aagttttttgggggggggggggaaaaaaataccccgggggaaaaaaaaaaaaatttttttttttcccccccaaaaaaaaaaaaggggtttttccccccgg
ggagaccccccctgggaaaaaaaaaaaaaagggggttttttttttggggcccccccaaaccggggggggaaaaaaaaaaggggttttttttttttgggcc
ccctttaaaaaaaaaaaaggggggggggccaaaaaacccaaagggggggttttttccccctttggggggggaaaaacccttttcaaaaaccccccccccc
ccccaaaaggggagaacccccccggttttttttgggggggaaaaaacccggggggggggcccccccggaattttggggttttaaaaaaaaagcctttttt
aaaaaaccccccccccaaaacccccccccccccttttttctaagggggggggaaattttcccccttttttaaaaaaccccccaatatttgggggccaaaa
aaaacccccccgtaaacaaaggggggggggaaaatcttaaaaacccccccggagaaaaaaaaactttttcccccccctttttttggttttgggaaaaaaa
ccctcccccccgcggtgaaaaccggagaacaaaaaaccgggaaaggccgccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: