XB-CLONE-4352445
Clone Name: IMAGE:7027399
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Gene:
rpl13
,
capn2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784244
[+]
taattaatccccccccccccgctttcttttccggccattttctccccgtagagggattaagtagccatggcccccagccggaatggcatgatcttgaagc
ctcactttcacaaggactggcagagacgggtagctacctggtttaaccagcctgctagaaagatccgcagacgtaaggcacgccaggtcaaagcacggtt
ggttgccccaaggcctgtttcaggtccagtcagaccagtggtaaaatgccctactatcagataccacacaaaggtgcgaatgggcagaggcttcagccta
gaagagttaaaggctgctggtatcaacaagaaagttgcccgcaccattggcatttctgttgacccaagacgccgcaacagatctacagagggcttgcaaa
caaatgtccagaggctgaaagagtaccgctccaaactaatcatcttcccacgcaaacctgctgcacccaagaagggagacagctcggctgaagagttgaa
actggctactcagttgaagggaccagtcatgccaatccgcagtgtgtacaagaaagagaagcccagagtcatcactgaagaggagaagaacttcaaagcc
tttgcaagtctgcgtatggcacgtgctaatgcccggctctttggcatccgtgccaagaaagccaaagaggctgcagaacaggatgttgagaagaaaaaat
aagttttcccgaactgttaataaattccaaacttggtgtaaaacatgttactttttattattttttaaatcagcagttcctctgccggcattcacctatg
ttgccaagggttggt
3' EST: CF784243
[+]atgaagctctctcgggtgggaccaccaccgaagggtttgaagatttcacaggtggacttgcagagtggtatgaactgaggaaggccccacccaatctgtt
caagattatccagaaagctctgaggactggatctctgctgggctgctctatagatatcacaagtgcagcggagacagaagccatcacattccagaagctc
gtgaagggccatgcctactctgtgactgcagcagaagaggtgcaatatcgagggaacctggaaaagctgatcagaatcagaaatccctggggggaagtgg
aatggaccggggcctggagtgacaattccagcgagtggaattctgtggatccggatgtgcgggaaaagttgatcaagagatgtgacgatggagagttttg
gatgtccttcaatgacttcttgagaaactattcaagactggagatctgcaatctgaccccggacactctggcctctgacaaatacagtaagtggagtctg
accaagatggatggcacgtggaggagaggctcgacagccggcggatgcaggaatttcccagaaaccttctggatgaacccccagtatgtgattaagctgg
aagaagcagatgatgatcctgatgataatgaggaaggctgcacctttattgtcggtctgatacagaagaaccgcaggaagcagaggaagatgggagagga
catgcacaccattggctttgccatctatgagctcactggccagaaggacattcgcctgagccgagattacatttctaggaaacagattcgggaaaaatcc
gacacctatataaacctgcgcgaagtga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: