XB-CLONE-4352421
Clone Name: IMAGE:7027391
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Gene:
slc16a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784260 [+]
taattatccccccccccccccaacagagagagacagccgggttatcggcagctacagattataccaaagagaaaccatatgaaaataaagagctttagaa
acttgaattcttttaaataatctaaacatttagtttcaagtctaccagatgcaagccttgaaaccaagcccccaaaggcactgaagaaacctaacccaga
gaggagttttgacctgagattttgacatcatgggaggagcagttgtggatgatgggccaactggcgtcaaggcccctgatggtggatggggttgggccgt
tctgtttggatgttttgtcatcactggattttcatatgctttccccaaagctgtcagtgtgttttttaaagaactgattagggagtttgggataggatac
agtgacacagcatggatatcgtccatcctactggctatgctctatggtacaggcccgctatgcagcatatgtgtgaatcggtttggctgtcgcccagtaa
tgatggttggtggtctctttgctgctcttggaatggtggctgcttctttttgcacaagtatcctcacaatttacctcactgctggggttatcaccggttt
gggccttgcactcaatttccagccctccctgattatgctgaaccgatattttgacaaacgacgcccactggcaaatggtctggctgcggcagggaagccc
cngtgtttctctgcgccctgtctcctctgggacaaaattcttcagtacaagttttggctggcgaggaggcttt
3' EST: CF784259 [+]tttttttttttttttatagtttaaaaaatttagaagtgtatatcatttactgacttttcagtgcaatgccaaataaaaccctgaaatcatatcacaaaaa
agtgtattcatctccataaataacacaaaaagacaagggaaaaaaaaataattaaaaaacacgcacacagttaccagttaggtttgagttaacattagtc
attggtaatagaagttatacagaccagcaaagacatggattgaaacactgtgtataaagtgaagctcgagctatctgtaggcttctgtgaaatggatata
caagtcctgtttttctcatgcagttggttgtatttgggctctctagctctccaaaaataacacgacttacaaaaaaaaaaaagttataaaaacaaacaaa
tgaaaccattaatgttctacactaaagggatttgcattttctgttacattaaaacaggcaaagtcctaatagcctacagcagccagtcaccggttagctt
ctccgagtctagtgcagataaaataatgacagcaatcagtctggttggcataggttaatggacttcagcaactggtttctacacaatgccttacagtttt
ctcgcaacaataaaatcaatgttcatttaaaacaattaatgattggcacagccctaaatagtctatcccctgaattaaatatcctcaagcttatcagact
gttcaaacacaggtctcaggattcgtcgcaacctctccgttcttctccttttcatccttcaagaactgctccacttctgctgaatccactttggncattt
tcagctgctgagccat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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