XB-CLONE-4352380
Clone Name: IMAGE:7027375
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Gene:
bloc1s2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080469 [+]
GGGAAGACAGGGAAGATCTAAGCTCGGAACTGCAGAGAACAATTCCCAGTGGCAAAGGAACCAAAGAAAGGTCCAGGTTATTTTACTACTATGGCTGAAC
AAGAAGGTCCAGACAAACCACCTTTAAATGAACAGCCTGTGGCTCCTTCTGCATCTCAAGATGATGCTACAGTGGAAACAGCAGAAGAAGCGGTTGAACC
TCCAGAGGCTGACATCAATGAGCTCTGCAGGGATATGTTCAGTAAAATGGCACTTTATCTCACAGGGGAACTAACAACTACCAGTGAGGACTACAAACTT
CTGGAAAATATGAATAAACTTACCTCTCTGAAGTATATGGAGATGAAAGATATTGCTGGTAATATCAGTCGGAATCTCAAAGACTTGAATAAAAAGTATG
CTAGTCTGCAGCCCTATCTTGAACAAATAAATCAGATTGAAGAGCAGGTGGCCTCTTTGGAAAATGCAGCCTACAAATTGGACGCCTATTCAAAAAGATT
AGAGGCAAAATTCAAGAAACTAGAGAAACGATGAAAGAACAATTTTTAATAGTGAAAATAGAAAGCACTGCAAAAAGAGCAAACTCCAGTATTACCTTCT
GCAAACCGCAGCCCAATAAGCCCCTGATTCTACAAAGTTTTACTCTGCTCATGTAATATAAACTTGATAGTAAAATAAAACAAACATTCCTTTCCAAAAA
AAAAAAAAAAA
5' EST: CF784287 [+]taattaatcccccccccccccgggaagacagggaagatctaagctcggaactgcagagaacaattcccagtggcaaaggaaccaaagaaaggtccaggtt
attttactactatggctgaacaagaaggtccagacaaaccacctttaaatgaacagcctgtggctccttctgcatctcaagatgatgctacagtggaaac
agcagaagaagcggttgaacctccagaggctgacatcaatgagctctgcagggatatgttcagtaaaatggcactttatctcacaggggaactaacaact
accagtgaggactacaaacttctggaaaatatgaataaacttacctctctgaagtatatggagatgaaagatattgctggtaatatcagtcggaatctca
aagacttgaataaaaagtatgctagtctgcagccctatcttgaacaaataaatcagattgaagagcaggtggcctctttggaaaatgcagcctacaaatt
ggacgcctattcaaaaagattagaggcaaaattcaagaaactagagaaacgatgaaagaacaatttttaatagtgaaaatagaaagcactgcaaaaagag
caaactccagtattaccttctgcaaaccgcagcccaataagcccctgattctacaaagttttactctgctcatgtaatataaacttgatagtaaaataaa
acaaacatttcctttcaaaaaaaaaaaaaaaaggatccactanttctagaacggcccgccaccgcggttggagcttggt
3' EST: CF784286 [+]tttttttttttttttggaaaggaatgtttgttttattttactatcaagtttatattacatgagcagagtaaaactttgtagaatcaggggcttattgggc
tgcggtttgcagaaggtaatactggagtttgctctttttgcagtgctttctattttcactattaaaaattgttctttcatcgtttctctagtttcttgaa
ttttgcctctaatctttttgaataggcgtccaatttgtaggctgcattttccaaagaggccacctgctcttcaatctgatttatttgttcaagatagggc
tgcagactagcatactttttattcaagtctttgagattccgactgatattaccagcaatatctttcatctccatatacttcagagaggtaagtttattca
tattttccagaagtttgtagtcctcactggtagttgttagttcccctgtgagataaagtgccattttactgaacatatccctgcagagctcattgatgtc
agcctctggaggttcaaccgcttcttctgctgtttccactgtagcatcatcttgagatgcagaaggagccacaggctgttcatttaaaggtggtttgtct
ggaccttcttgttcagccatagtagtaaaataacctggacctttctttggttcctttgccactgggaattgttctctgcagttccgagcttagatcttcc
ctgtcttcccgggggggggggggaattatttaacccnagtgcaaaaaggaacaattagctttgttattcggccccgggataaaaccgaagttgccagttg
gaccttgggaagatggacaccctggtgggagaaaaaaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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