XB-CLONE-4352368
Clone Name: IMAGE:7027371
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Gene:
ilf2
,
rps4x
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784295
[+]
taattaatcccccccccccccccacacattgcagacgcagccatggctcgcggaccgaagaagcatttgaagcgcgttgctgcgcccaaacattggatgt
tggacaagctgactggtgtctttgctcctcgtccatccgctggtccccacaagctccgtgagtgtctgcccctgatcatcttccttaggaaccgacttaa
gtatgcattgactggggatgaggtgaagaagatctgcatgcagcgctttattaaaattgatggcaaggtccgaacagacatcacatatcctgccggcttt
atggatgtcatcagtattgagaagactggtgaacacttccgtctggtttatgataccaagggccgatttgcagtgcatagaattacagctgaagaggcca
agtacaagttgtgcaaggtgaggaagacatgggtgggaaccaaaggcattcctcatctggttacccacgatgcacgcacaatccgctaccctgatccttt
aataaaggtcaatgacaccatccaaattgacctggaaactggcaagatcacagacttcattaagtttgatactggtaaccttttcatgggtgactggaag
agccaacttggggggaaattggtgtcatcccccaccgagagaggcacccaagctcatttcaatggggtccctggtcaagggattgcaaatgggaacaagc
tttgccccccagggcttaatcaacattttttggtcatttgggcaaagggcaaacaaaccccctgggatctcctatttggcccccggggagaaagggggta
tcccttccct
3' EST: CF784294
[+]gcgtccttagggtctcgaccaccttaatgcccaaagcagaaacggcttccagtgtgggaaggatctttaggatgacgaccaagtctgcaacattatggcc
tgtgctcatggtccctttcttgtaggagcccacctgtcgcacctcttcaatttgcacttcaaagtttcccggggctacgatcaaattgtcgataacgttg
ttgattttggtgaccaaagaaaggattgaggcctgctcggctgtgctgggggccagatcttggtttcgtttgagcagtgactcgctgaatgagctatcgt
cgggggcgggtttcacacgcgggaatgccatttcacacacgtggaaatcaaagggaatgtgagggacgaagggtctgaaacctgtgttaggtcccactct
ggagccaaagcggccacgtccacgtcctcgttcacccctcatgtttcagatcttcgttcctcacactcccgcacaatggacaccctctcggccctcgata
cagccacactggggggggggggggggggggggaaaaaaaaaaaattctttttgttcccccccaagtgtttctcccaccctaaggaaaaatgttgtattgg
gcccccctccccttgaaatttttcatgcgccggcaaaacatgcgccctttggtattataaaaccaaaaaggaaaggtgtctcaccacatctcttctccaa
aaatggagggagacccctccatataaccccgcgggtgaatatatgtaaatttcttagttttcaaacaccttttcctaatcaatatttttttaaaagaggc
ggcgcggggggaaaaataattttcttccacccaccctccccaccaagaaaagagattaatattataaagggggtccaccaaaaaagaaaagaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: