XB-CLONE-4352299
Clone Name: IMAGE:7027347
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Gene:
rpl9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784340
[+]
taattaatcccccccccccccgccctttcctctgtcagctgcgagaatgaagaccattctcagcaaccagattgttgacatcccagagaatgttgacatc
tctctgaagggccgtacagttactgtaaaggggcccagaggtgtcctgcggaaaaactttaaccacatcaatgttgagctgtgtcttctgggcaagaaga
agaggaggctccgggttgacaaatggtggggtaacagaaaagaactggccacagtgcgcaccatctgcagtcatgtgcaaaacatggtgaaaggagtcac
actgggctttcgttacaaaatgagatctgtgtatgctcactttcccatcaacgttgtcattcaggagaatggatctcttgtggaaataagaaacttcttg
ggtgaaaagtatatccgcagggttcgcatgagatcaggtgttgcatgcgccttatcccaagcccagaaagatgagctgattcttgaaggcaatgacattg
aacttgtatcaaactctgctgccttgatccagcaagccaccacagttaagaacaaggatatcacaaaattcttggatggtatctacctatccgaaaaggg
tacagtacagcaggttgaagaataaaaccctgcaaaagccgggttcccgggctgttcccaaaaaaaaagtcccctttaactaaaaaaaaaaaaaaaaggg
gttcccctagtttctagaagccggccccccacccgcggtgggaatcttgggccggtggcccccaaccttggtttttattggcagctcccacttttgggtc
tcccccatattaatagcacatatacctcccccccaaaattttttccattacctatataaggcgcttttttt
3' EST: CF784339
[+]tttttttttttttttagataaagggactttatttttggaacagcacaggaaccagacttttgcaggcttttattcttcaacctgctgtactgtacccttt
tcggatacgtagataccatccaagaattttctgatatccttattctttactgtggtggcttgctggatcaaggcagcagagtttgnatacagttcaatgt
cattgccttcaagatcagctcatctttctgggnnctggataaggcgcatgcaacacctgatncccatgcgaacccttgcnggatatacttttcaccaaaa
aagttccttaatttcccaaagagattcaatttcccctgaatgaccaacctttaatggggaaaatggagccttaccccgatttcccattttttaaaacaaa
aacccccggggggccccccttttcccccggttttttcccacaataaaccgccaaaaaggggggggccccttgtggggcccaattttttttttttttgttt
tccccccccccatttttttttttaacccccgggggaaacccccccccttttttttttttttttggtgcgcaaaaaaaaaaaaaaaacccccccccccacc
acctggtgtgggggggggaaaaaaaaaaatttttttttccccgggaaaggaaaaaaacaaactggtgggggggcccccctcttttattaaaaaaaaaaga
agagggggggggcccccccccctccttaaaaagaaagaaggcgccgccccccccccttctccgggggggggggggaaaaaaaaaacaaacaatttttttt
tttataaaaaaaaaaaaattttttcccttctcctcccccccccgcggcgggggagagaaaggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: