XB-CLONE-4352296
Clone Name: IMAGE:7027346
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Gene:
hmgb3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784341
[+]
taattaatccccccccccccctaaagagaaatcaaagtttgacgatctggcaaaggcagacaaagttcggtatgacagagaaatgaaagactttggacct
gtaaagaaaggcaagaagaagaaagatccaaatgcccctaaaaggccaccatctggctttttcctcttctgctcagaattccgtccaaaaataaaatcta
caaaccctggtatttccattggtgatgtggcaaagaagctaggtgagatgtggaataaccttaacgatggtgagaagcagccatacaacaacaaggcagc
taaactcaaggaaaaatatgaaaaggatgttgctgattacaaatctaaaggaaagttcgactgtgctaaaggagccccaaaattagcacggaaaaaagag
gaagacgaggatgatgatgatgaagatgaggaggaggatgaagaggacgaagaagatgatgatgattaaagttgtacattctgtccatgtgaataccata
gagtaggggaaccgaagtcaatgactccatcttaaataaggtggtcaacttgccctcattaggtttaattgcaaaatttggttatggaatgtttcataat
gatgctcttgggattgtaactggtttacattaagtagcagtgcgtgttaacatgagcaatatgaaactgtcaaagctgtacatatttccaaacatttttt
aaagaaaaaagcgctctagtgttctcctcactctgtgcactttgctggtagtgtaacaaagcattttaaaatgtttcaagcatttttttatttaaggtgt
ttactaa
3' EST: CF784342
[+]tttttttttttttttattttttttttttttttttaaatgtaatactgttttatttttgcttcaaaaacatttcagcattctaaacataaaaaacataaca
atgcaaatataagtacagaacgtttttgaacttgcagatcctgtactcactcgctcttcacttcagtcagtaaagtagtgtctacagtctttgtttaaaa
aactaccgcacttaactaaaaaataaacccataaatgtttctcatgccagctgaaccccttccgacaactaatggcagaatgctaatctcactatataca
gaaagacaactttaagctaaatagacgcccaatgtagtgtaagttgatttcatgtgcatgctttgggctagaaggacaaccccctttgaaaagggtgacc
tagtccagggaagtttgtaaaccctgaggtaccaagagctccaagaaatatgcaccataggaatttgtctagtttacagattacagatatgtacaagtca
taacccaggattttctaatcaataaccattaagtaacaccttaaataaaaaaatgcttgaaacatttttaaatgctttgttacactaacagcaaagtgca
cagagtgaggagaacactagagcgctttttttctttaaaaaatgtttgggaaatatgtacagctttgacagtttcatattgctcatgttaacacgcactg
gctacttaaatgtaaaccagttacaatccccaagagcatcatttatgaaacatttccataacccaatttttgcaattaaacccctaatgaggggcaaagt
tgacccaccctn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: