XB-CLONE-4352285
Clone Name: IMAGE:7027342
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Gene:
srp54
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784348 [+]
taattaatcccccccccccccgggaatggtgacgtttcatatagaacggaagttcccgtgctgtgaaattcctgtcggtctgttgagtttgtaatttcgt
tatatggacattgtgttgcctgagcatcgaccggtccagttggggatataaaagcatatagtgggagggctgagggacccccacattgggggcttatttc
tcatcaggtgttgtgtaacaaacagaagccaagatggtgttggcagacctaggacggaagattacttcagcactgcgctcactgagcaatgcaacaatca
tcaatgaagaggtgctcaatgctatgctcaaggaagtatgcaaggctttactggaagctgatgtgaacataaagcttgtaaagcagctgagggaaaatgt
caagtctgctattgatctggaggaaatggcatctggcttgaataagaggaaaatgatccagcatgctgtcttcaaagagctcgtcaagctggtggatcca
ggggttaaagcatggacaccaactaaaggaaaacctaatgtcataatgtttgtgggtttgcaaggaaatggaaaaaccaccacttgtacaaaattggcat
attattatcaaagggaaggatgggaaaacctgttttgaaatgtggctgacacttttcagagcctggagctttttgacccgtttaaaacaagaatgccacc
aaaagcccggaaataccttttctatgggcagtttttacagaaatgggatcccccttattttctaaaagcgggcccgccacccacgggtggagagcttt
3' EST: CF784349 [+]tttctgtataactgccatagaaaggattctggcttttgtggcattctgttttaactggtcaaaagctccagctctgaaagtgtcagcacatatcaaacag
gttttccatcctttcctttgataataatatgccaactttgtacaagtggtggtttttccacttccttgcaaacccacaaacattatgacattaggttttc
ctttagttggtgtccatgctttaacccctggatccaccagcttgacgagctctttgaagacagcatgctggatcattttcctcttattcaagccagatgc
catttcctccagatcaatagcagacttgacattttccctcagctgctttacaagctttatgttcacatcagcttccagtaaagccttgcatacttccttg
agcatagcattgagcacctcttcattgatgattgttgcattgctcagtgagcgcagtgctgaagtaatcttccgtcctaggtctgccaacaccatcttgg
cttctgtttgttacacaacacctgatgagaaaatagcccccaatgtggggggtccctcagccctcccactatatgctttttaaatccccaactggaccgg
tcgatgctcaagcaacacaatgttccatataacgaaatttacaaactccacaagaccgacaggaattttcacaagcacgggaaaacttccgttcctatat
gaaaacgttcacaattccccgggggggggggggggaattaaataactcccaagtgcaaaaaaaaacaagtaacctttgtattctgggccccggaataaaa
acccaagttggcatttggaaccttggggaatgggaacacctcgtggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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