XB-CLONE-4352266
Clone Name: IMAGE:7027335
|
Gene:
ppp2ca
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784361 [+]
gcaggttatnacatttggacaggatatatctgaaacattcaaccatgctaatggccttactttggtttcaagagcccatcagctagtgatggaaggatac
aattggtgccatgatcggaacgtagtaaccatttttagtgctccaaattattgttaccgctgtggcaaccaagctgcaataatggaacttgacgacacac
taaaatattcattcttgcagtttgacccagcaccacgcaggggagagccacatgtgacccgtcgcacacccgactacttcctgtaaacagaattttactc
ttgtacagtattgctaccagcaggattttgtcatgagatctcggaaaagcagcagtaacttcaaattcaggggagcgttgtcacttatgtactcttaaat
gatggaccaaaagatgtgccataaaagcctcttgtttcaacagcccgtgaccaccttagagaccctgtccttgcatgctgaagtggcattgtctgtcnaa
gagaaaaaaaaaaaaaatctgtttcctggcatagcactacttgtagtacatttgctttctcggagagactgcaaaaactaaaacattaacaacccgtgaa
tacaataactcccgttagctatagctctaccgggcgtgatttttggatagatagaaaccagccggaattaccccttcttgtgctgcagggggaggcaaaa
gcatttcgggcggcttctctgggggggaaaggggatccttgggaattttttaatcaaggg
3' EST: CF784360 [+]tttttttttttttttaaggactttgttcacaagtttattatgggggaagggaaaaagaaaggatactgctgcactgtcctgcagcaaagtcttacagaag
atctggttccggtacagatgtatttgttattaactacagaaaataaaacagtcgcagacttgaaaattgatgcggaagggtgaaacgaaaaaaaaatagt
aagcgcatatgaaatttaaattaaattgtattccctgaaaataaacatatctgaagtcacaataaaggcagcagggcccttggtacgcattgattaaaaa
cgtccatgtatccccttcacaccagagaagacagcagaatgctgttgcctcccctgcagcacagaaggggttaatccgactgtttctatcttatctataa
tcatgccaggtagagctatagctaactggagttattgtattcacgtgttgttaatgttttagtttttgcagtctctccgagaaagcaaatgtactacaag
tagtgctatgccgggaaacagattttttttttttgtctctttgacaaaacaggccacttcagcatgccaggacagggtctctaaaggggtcacgggctgt
tgaaacaaaagggttttatgggacatctttttggtcccttcttttagaaggtacttaaatggcaacccctcccccggaaatttaaaattaatggccggct
ttttcggaagaacctcatggaaagaaatcccgggggggggagcgcaataggtggtatggatatataagaagttccttgtttataaaaggaaagttaattc
cgggggtgggggcggaccggggttcacattaattaggtctctcgtccccgcattagaggaggggatcatgtgagaactaaagatatataa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: