XB-CLONE-4352253
Clone Name: IMAGE:7027330
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784368
[+]
taattaatcccccccccccccacccccccttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaagga
aaagactcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaactggacatcttatctacaaatgtggtggtatcgacaagaga
accatcgaaaagttcgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggta
tcaccattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatcac
tggtacctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgcc
ctccttgcctacactctgggagtaaagcaactgatcgttggggttaacaaaaatggattcaacggaaacccccatacggcccgaaaaagaattgaggaaa
tcgttaagggaattcagcccataccttaagaaagattggttacacaccctgaaacgggtgccttttgtaccctattttcgggagggaaacgggtaaaaaa
aggctttgagcccaagcgccaaaaaatgcttgggttttaaggggggggaaaatcctccccgtaaagaggggatctgggcagacgcaaccaccccctgg
3' EST: CF784369
[+]tttttttttttttttcttttttttttttttttttttatcttttaactttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatt
tagaaattactggatttaaaacactaataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgt
aatcattaaccagtctttgactattaaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagat
gctggaaatattcatttgcctttctgtgctttctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacag
caacagtctgcctcatgtcacggacagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaat
ggcagcatcaccagacttcaggaacttgggattgtcttccagtttcttaccagaacggcgatcaatcttttccttcagctcaacaaacttgcaagcaatg
tgagctgtgtggcaatccaacacagggggcatatccagcacccatctgggcctggggggttcaagaaagaataacctgggctgtaaaaggaacccgcttt
ccattgggtgggtcattcttggctgtcaccaagcaacagttgccacgaacggaggtcctttcaagaaaacgttttttcacgtttaaaacccaacct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: