XB-CLONE-4352247
Clone Name: IMAGE:7027328
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Gene:
rpl5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784372
[+]
taattaatcccccccccccccgcttttcccagtgctacagcgccctaacgtttgggctgtgactctccggtcctagccccgacgggacaaaatagagcaa
aatggggttcgtaaaggttgtcaagaacaaggcttattttaagaggtaccaggtcaaattccgcagaaggagagagggtaagactgattactatgctcgc
aagcgtttggtgatccaagataagaacaagtacaatactcccaagtacaggatgattgtacgtgtcaccaacagagacatcatctgccagattgcttacg
ctaggattgagggagacatgattgtttgtgctgcctatgctcacgaacttaccaagtatggtgtcaaggttgggctcacaaactatgctgcagcatattg
tacaggcctgctgctggctcgcaggcttctcaacaagtttggcctggacaaggtttatgaaggccaggtggaagtaactggagatgagtacaacgtagag
agcattgatggggaacctggtgccttcacatgctaccttgatgccggcctgacccgaaccaccactggaaacaaagtttttggtgctcttaagggagctg
ttgatggaggtttatctattccacacagcaccaagcgtttccctggctatgactctgaaagcaaagaattcaatgctgaggtccaccgcaagcacatctt
tggccagaacattgctgagtacatgcggccttctgacggaagaagatgaggatgcgtttaaaaagcagttctctcagtacataaagaaatggtgtcacaa
caaaa
3' EST: CF784373
[+]ttttttttttttttttgatgttaatagatttatttttttgctgcaaatacttagaaaatcgcaacataatccatttagctgtctgtcttctcctgagctc
tgaggaaactggcttttttctgagcaacacggtcctttttctgtgccagagacaatttggcacggttccacctcttctttttgacttccttcttgggttt
cttttcgtgcactggattctcccgaatacctgcatgggcctttttgtaaatgtcttccatctggtctgctgtgacaccattctttatgtactgagagaac
tgctttttaaacgcatcttcatcttcttctgtcagaaggcgcatgtactcagcaatgttctggccaaagatgtgcttgcggtggacctcagcattgaatt
ctttgctttcagagtcatagccagggaaacgcttggtgctgtgtggaatagataaacctccatcaacagctcccttaagagcaccaaaaactttgtttcc
agtggtggttcgggtcaggccggcatcaaggtagcatgtgaaggcaccaggttccccatcaatgctctctacgttgtactcatctccagttacttccacc
tggccttcataaaccttgtccagggccaaacttgttggaaaacctgcgagccagcagcaggcctgtacaatatgctgcagcatagtttgtgagaccaacc
ctgacaccttacttggggaggtcgtggacctaggcagcacaaaccaacatggctcccctcatcccaagcgtaagcaatctggggaaagaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: