XB-CLONE-4352239
Clone Name: IMAGE:7027325
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Gene:
XB5842389
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784378
[+]
taattaatcccccccccccccgaggtactgcagggggagaagaaagaagccggacattaacccaacccactgacaggatgaagtgctttgtggtcctgct
ggtttgcatctccattgggtgggtccattctggcaaaataaagccgaaagttaatattgcaagattgggagaagccacacagagctcaacgtacagaccc
gagtacaacgctgccgccgctattgatggtgatactgactctaatatgatggcgggatcatgttccctcactggttatgacaacccggcttggtggcggc
tggacctgaagaagaggtacaaggtggagaaagtggtgatagtgaacagaggggactgctgcggcgagcgcctgttgggagccgaggtccgtgttggaaa
tacagcaaatgtgaacaacccgacatgtggagccataaccgacgtctccaaagaagccatcactgtggtctgccatggaaaggagggtcagtatgtctct
gtggttattcctgggcgtgaagaatatctccagctctgcgaagtggaggtctatgggaaagaatatagaaaaccaaaccatgacaattcaaaatgactca
gtactggaagacataggcaatttattcaatttaaggctgaagaagactttttgctcattaaccctgaatgaaatctgcttgtgaaacattaaataccaat
ggctgggttcaaaaaaaaanaaaaatatttcaaaaaaaaaaaaaaaaggatcccccttatttcttaaaccggcccgccaaccgccggtggaaacttgggc
cg
3' EST: CF784377
[+]tttttttttttttttattttttttttttttttgaaccagcatttgtatttattgtttcacagcagattcattcaggttaatgagcaaaagtcttcttcag
cctctaattgaataaattgcctatgtcttccagtactgagtcatctgaattgtcatggtttggttttctatattctttcccatagacctccacttcgcag
agctggagatattcttcacgcccaggaataaccacagagacatactgaccctcctttccatggcagaccacagtgatggcttctttggagacgtcggtta
tggctccacatgtcgggttgttcacatttgctgtatttccaacacggacctcggctcccaacaggcgctcgccgcagcagtcccctctgttcactatcac
cactttctccaccttgtacctcttcttcaggtccagccgccaccaagccgggttgtcataaccagtgagggaacatgatcccgccatcatattagagtca
gtatcaccatcaatagcggcggcagcgttgtactcgggtctgtacgttgagctctgtgtggcttctccccatcttgcaatattaactttcggctttattt
tgccaaaatggacccccccccatggagatgcaaaccagccgggaccaccaaagcactttcatcctggtcaatggggttgggtttaatgtcccgggtttct
tttctttctccccccttgcaataaccctccgggggggggggggggggaatttaattttaacctccgaggggccaaaaaaaagaaacaagttaagccttgg
ttatattccgggggccccgggaattataaaacaccgcaaggtgggcctcatttttggagaaccccgtt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: