XB-CLONE-4352233
Clone Name: IMAGE:7027323
|
Gene:
dnaja3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF784382
[+]
aattaatcccccccccccccattgcgggcgggccgcggatccctagcaggcccctgtgagcggctgtcccagttccataccagctcttcctcatacaaca
agacagatttttaccaggtactgggggtgccaaggaacgcaagccagaaagaaataaagaaggcctattaccagctggccaaagagtaccatccagacac
taacaaggaggatccgcaagccaaagaaaagttctctcagctggcagaagcatacgaggttttgagcgacgaggttaaacgcaagcagtatgacacttat
ggaactgcagactttgctgctggggcagcagggggaggaggacaacaatactggagaggaggtcctactgttgatccagaggaactgttccgaaagatct
ttggggagttttctggctccccattcggagacttgggtagcatgtttgagcagccgcaagagtacatcatggatctcacgttcattcaggcggctaaggg
agtgaataagcagatctctgtaaacattacggacacgtgccagaagtgtgatgggaaaggaaatgagcccggcaccaaactgcagcactgccattactgc
aatggaactggcatggaaactatcaacactggcccctttgtgatgcgctcaacatgcccggcgctgcggtgggaaagggagctaccaggaccaacccttg
cctgtcatgcagaggctctggccaaacaaagcaaaaaaaactgtttacggggcctgtgccctgctggggtgga
3' EST: CF784381
[+]tttttttttttttttagattgttacaagaacaagaatggaatagatggtctgtatggcagatgcctccttcattcaaccttaacgtcagtccttggttat
tttgtgcccgagcaaggtgcaactcaatatggcttcttctggctgcctggcaaatgtttatgcttattctgtatggtgcttcatggcatagtggtgaaaa
gagtccatagatttgctccattttcacagacaggtaagctctgcaataactagtttcctgttgatcgttttcctcactgaggtgtcaggagctaaacatt
ttctttaatttgcttaggaatccctccttgttctcccctgcctccttagtctcagggctctcctccccagttgcagatctgtgctggtttctgcctgttg
ttgtgttagtgatgccatttactgttccttctacatctgtctcatcttctgcaaagctaagcattagcatccgttgcctgtcagtcaaatgcctgggtac
tctgaccttgatgtggatatagtgatctccaaagccataactgttcatacgagggatacctttcccactgatccgaatcctctggtctgcttgtgtgccg
gcagggacagggacattgatgggatcataaagtccttgtgcacgagccgaaaccccccagaacagcttgagcaatggaaatatacaggtcccgagtgaat
atctgccccatcccttctaaagaatgggactttttctgcacccgaaaatgtaataaaaattctcttttctttcccacccgggcatcccaaaattgtctgc
cccgtctttccaaaccaagcagggcacaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: