XB-CLONE-4352216
Clone Name: IMAGE:7027317
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Gene:
acta4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784393 [+]
taattaatccccccccccccgagcgcacagctactcctactctctccctgctgcaaaagtctcagtccctctccagactcaaacatgtgtgacgacgatg
agaccaccgctttggtgtgcgataacggttctgggctagtgaaagcaggctttgccggagatgatgctccccgtgctgtcttcccctcaattgtgggacg
tcctcgtcaccagggtgtcatggttggtatgggccaaaaggactcctatgttggggatgaagcccagagcaagaggggtattctgaccctcaagtaccca
attgagcatggtatcatcaccaactgggatgacatggaaaagatctggcatcacaccttttacaatgagttgcgtgtggccccagaggaacaccccaccc
tgctcacagaggcacctcttaaccccaaagccaaccgtgaaaaaatgacacagatcatgtttgagaccttcaatgttccagcaatgtatgtagccatcca
ggccgttctgtccttatacgcatctggacgtaccactggtatcgtcttggactctggtgatggtgtgacccacaacgtgccaatttatgaaggttatgct
ttgccccatgccatccagcgtctggaccttgccgggagtgaccctgactgaactacctcatggaaaatccgaatgaaaaagggtaattcttttgtcccaa
caccttgaaagggagaattgtgaggggacatctagggaagaaaccttggcttatggggggctctgggacctttgaaaaaatgagaaaggggnaaaaggca
acccacatttccctcattctttcgggaagaaaaaaaccttttgtaaacctccccacc
3' EST: CF784392 [+]tttttttttttttttaatgtttataacaaatgttttattgtaattttgtatatatttgtgacatttgtacacagaggaattggggtaagaatataattag
aagcactttctgtgcacaattgaggggccggcttcatcatattcctgcttgctgatccacatctgttggaaggtggagagagaggcaaggatggaacctc
caatccatacagagtatttacgttctggaggagcaataatcttgatcttcattgtgcttggagcaagggctgtgatttccttctgcatacggtcagcaat
accagggtacatggtggtaccaccagacaaaacattgttggcatagaggtccttacggatgtcaatgtcgcacttcatgatggagttgtaagtggtctca
tggatacctgcagactccataccaatgaaggatggctggaagagtgtttctgggcaacggaacctttcatttccaatggtaatgacctgtccgtctggga
gttcatagctcttctccaaagatgaggaagtggctgctgttgccatctcattttcaaagtccagagccacataggcaagcttctccttaatgtccctcac
aatctccctttcagctgttgtgacaaaagagtagcctctctcagtcaggatcttcatgaggtagtcagtcaggtcacgtccggcaaagtccagacgctgg
atggcatggggccaaagcataaactttcataaattggcaccgtgttggggtcacaccatcacccaaagttccaagacgaaaaccaggtggtaacctttca
agaatgccctataaaggaacaaaaccgggccctggaatgggccttacatacaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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