XB-CLONE-4352205
Clone Name: IMAGE:7027311
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Gene:
rpl8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784400
[+]
taattaatcccccccccccccgctcttcgcgtacagccccggcctagtgcacaatgggacgtgtaatcaggggacagagaaaaggtgcaggctctgtttt
taaagcccacgtcaaacacagaaaaggtgccgctaagcttcgggctgtcgacttcgctgaaagacatggctacatcaagggtattgtgaaagacattatc
catgatccaggccgtggtgctcctcttgccaaggttgctttccgtgacccatacaggtttaaaaagaggacagaattgtttgttgcacctgatggaatcc
ataccggacagtttgtgtactgcggcaagaaagctcacttgaacattggcaatgtactccctgttggcaccatgtccgaaggcaccatactttgtcgtgt
ggaggagaaaacaggtgatcgtggcaaacttgcccgtgcctctggtaactacagccaccgattatctcccataaccttgaaacctaacaatattccgatt
tgaaccctgccctttctgtgctcccagaaagggccatttctaatcctgcaaaaaaggaatcttattcgtttgggggtttgttttgctcgggcggttgggt
ccccttttttgcaccaaattccccatcccttttaaacggtttatgtacctccggccattaacccccttacagtacaccaaaaggcgtaaaaaagaagaac
atcattggctttggcctcccccccgtaaaccatagagaaatgagggggcactaaattaacaccctcttgccctaaaaaaccacttatattttccaccgcg
cgtgggtgggttaccacccctatttttatcccgtcaatccggattatattatcctcccccttgttaattttctccgcctagtgtaatgagaacaatgccc
cccccctatatttgtgttgcacccttttatattcacacccgtagtccacatntttatattatggttttttgctccgcgcacgtggcgaaaacacgaagga
tttttgatatgttgccgctagcgggtgcgcctattagaatagatagaactgcgccggcgccaccatatagttgatttgctat
3' EST: CF784399
[+]tttttttttttttttctttttacaatttatttggaagttatcagatagacgagctttagttctccttttcctgcactgtctttgtaccacgcagacgacc
ggtacgacgggcagcaataagaccaactttgcgaccagctggggcatctctcctgatggttgagggcttaccaatatgttggtggttaccaccaccgaag
ggatgttctacaggattcatagccacaccacggacacgtggccagcagtttctctttgccttgtatttatggtatgcacgtcctgccttcaggatgggtt
tgtcaatacgaccaccaccagcaacaaccccaacaatagctctgtttgcagatgagatgaccttcttggagccagaaggcaacttcactctagttttctt
tgtttcaggattgtgggagataacggtggcgtagttaccagaggcacgggcaagtttgccacgatcacctggtttctcctccacacaacaaactatggta
ccttcaggcatggtgccaacagggagtacattgccaatgttcaactgagctttcttgccacagtacacaaactgtccggtatggattccctcagctgcaa
caaacaattctgtcctctttttaaaactgtatgggtcacggaaagcaaccttggcaagaggagcaccacgggcctggatcatgggataatgtcttttcac
aataccccttgatgtagcccatgtctttccagcgaaagtcgaacagcccgaaagctttagcggccaccctttttctgttgttttgaacgttgggctttta
aaaaacagaagccttggcaccttttttcttcttgtccccctggattacccccctccccactt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: