XB-CLONE-4352197
Clone Name: IMAGE:7027308
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784405
[+]
taattaatcccccccccccccgaggtactgcagggggagaagaaagaagccggacattaacccgacccactgacagtatgaagtgttttgtggtcctgct
ggtttgcatctccattgggtgggtccattctcgcacaatctttcagaaagataatattgcaagatcgggagaagccacacagggctcaacatacagaccc
gagtacaactctttttccgctattgatggtaatactgactctaatatgaggtacggctcatgttccctcactggtaatgacaacccggcttggtggcggc
tgggcctgaagaagaggtacaaggtggacaaagtgctgatagtgaacagaggggactgctgcagcgagcgcctgttgggagccgaggtccgggttggaga
ttcagccgataataacaacccagtatgcggcactgtaaccgacgtctctgaactatacatcactgtctcctgtcatgggaaggagggtcggtatgtctct
gtggttattcccgggcgtgaagaaactctccagctctgcgaagtgaaggtctatgagtcagaaattccctctatttcactgttccgcctaattcgtattg
gttaaagaagtggttaaagaagacttttgctcattaacctgaatgaatctgctgtgaaacaataaatacaatgtgcagttccccctattaatttatcacc
ccattcatctaattaccaatatattttgcccccaattactcaccccatctttttcaccccccctttttatatgcttcattaagaggggcctccctttatt
tttcataaggggggggcccctcacacctgggggcgaaagatattttggggcgcggggggcccaaataatgtc
3' EST: CF784404
[+]tttttttttttttttatttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttaaaaccc
ccctttttttttttggttccccccaaaattctttcggggtaaagaaaaaaaattttttttaaccccttttttaacaaaaaaaaattggggggaaaaggaa
aaaaaggggattttttgacccaaaaaccccccctcccaaaaagggggagatttttccccccccgggaaaaacccaaaaaaaaacccccccccccccccct
ggagggggaaaggggggttttttttaaaaaacccgggtaaaagggccccaaaggggggggtttttacgggggaaaaccccacccgggacccgggccccca
aaaggggccccggggaaaaaccccccttgtttaaaaaaaaaattttgcccccctgggacccttttttgggggcccccccccccaaagcgggggggttttt
ccccgggggggaaaaagccccccccccccattaaaaggcggttttccccccaaaaggggggggcccttttaacccgggttttttttttaacccccggggg
ggccccccccatttggaaaaaaatttttttaaaaaaattggggaaaaaaggcccccccccggggggggggaacccccgggggcccaaaaaacttttaatg
ggcccggggggcggggtaaaaaggggggttttttttttccccccccgaaaaacccggggggggggggggggggggggataaaaatccccgggggggaaaa
aaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: