XB-CLONE-4352191
Clone Name: IMAGE:7027306
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Gene:
impdh2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784409 [+]
taattaatccccccccccccctttcatctcacagaatggaaaagctgaatgattaccattttttatgtcccatttgactgcaaataaacactgaaacatg
tataaattaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
gggttcccctttttttaaagggccccccccccgggggaacttggcccgggccccacttttttaattgcacctaaaaagggtaaaaaaaaaacaaaaacct
tccaaattttcaaaaaaaaacatttttttccccgccatccaattgggggttggccaaacctctcaaaggaattttaccgggccggaatgggaaataaatt
cggccaacccccaggccggaaaaaaccctcaaaaaaaaaaactggttaaggtacctttgaaggggaaaaaaaccccttgagaaaggggtgccattaaggg
ggggaaaaatcccaaggctccccaccggggaaaattttcaaaacttggctttttttttattcaccaaacctggggtgtgaaaattccccagccctcccta
aaaagggaaaaaaatttccaaaagccctgcctcttaaatttattcgcccaaccaaaaattcccccccccctaaaatccgcccccaatccccgccccaaaa
aaatccccaccacaaattttcccgcccattttctccccccccaaaggtggggagaaaaaaatattttttttccatcccacccacaaaaggcccaaaagcc
ccccccctccctaccttttagaagatttttttcaccaaaaaaattacgggaaagagagtttctttctcttattgaagagaaaaatacaa
3' EST: CF784408 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttaaattaaac
ccggttcccgggtttttttccccccaaaggggacaaaaaaaaggggaaccctccccctttccccttctggaaaaaaaaagggggggggggggattaatta
cccccggggcaaaaaaaaaaaattccttgttttccgcccccaaaaaaaccaaggggcctttggccccgggaggggacccccggggaaaaaaaggcaaagg
ggatttttttctaaagggccccccaaacccgaggggtaaaaaacaaaagggttttttttaattggacccccctttaaaaaaaaccaagggggggggccca
aaaaccctaagggggggattttcccccccctggggcgggggaacccccgttccaaggccccctccccgcccccaaaagcgggaacccccccggggtcttc
ctggggggccaaacccccggggaggggcccccccggcaatttgagggttcccaaaaaaacccccgctaaaaaaaacccccccccgaacaccccaaccccc
cttttgcccaagggggggggaaattgttacccacttttggaaaaccccccggtaaatttggggccacaaaaaaacccccctttgacctcaaggggggggg
gaaaaacttgaaaaaccccccgggggagaaaaaccccttttcccccccccccatgggggttcgggccaaaaccccccccccccgtgggggaaaaacccga
gggaacaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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