XB-CLONE-4352183
Clone Name: IMAGE:7027303
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Gene:
dpy30
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082731 [+]
AACTTCCGCTGTTAGACACGCGTGGCCACGCCCACAAGGCGGCTACGTGATGCGTCACTGCGTTACGTATAGTCTCGCGTGATGAGACTTGTGGCGTTGG
GATATTCTGTAAGAGAGGAGGCTAGGAACCTGCAGTAAACAGTTTGCGGGAGGTGCGAACGCTTCCTGTTTTAAATCAGTGTTACCATGGATACAGAGCA
AAGCATGGAGGGGCAAACACAGGTTTCTGAAAACCCTCATTCCGAATATGGGCTCTCAGAGAATGTCGAGCGAATAGTGGAAAATGAGAAGACCAGCACG
GAAAAAATCTCCAAACAGAAAGTTGATCTGCAGGGCTTACCTACAAGAGCATATCTTGACCAAACAGTAGTTCCTATACTTTTACAAGGCCTTTCAGTGC
TTGCAAAGGAAAGACCACCAAACCCAATAGAATACCTTGCAGCTTATCTCTTAAAGAACAAGACACAGTTTGAGGAAAGAAGCTAGAATGGATACATCAG
CCTCCTGCATTTTACATAAATATTGATTATTAAAGAGATTGCATTTCGTCCACAGTAACTTAAATTTACCAATTCGTGTTTCCAGCAAAAGTGAAAATTC
CATTTAGACTGTCACATTGGGGTGAAAAGCAAGACAAAGGGAAGGGGTTAATTTCTTGCAAATTTATTGTTTTTGACCAGATTTCTGTTTTGTTTGTTTT
TTTAAATAAAAATGTGACTGCAAATCTTAAAAAAATGAAAAAAAAAAAAAAAA
5' EST: CF784414 [+]taattaatcccccccccccccaacttccgctgttagacacgcgtggccacgcccacaaggcggctacgtgatgcgtcactgcgttacgtatagtctcgcg
tgatgagacttgtggcgttgggatattctgtaagagaggaggctaggaacctgcagtaaacagtttgcgggaggtgcgaacgcttcctgttttaaatcag
tgttaccatggatacagagcaaagcatggaggggcaaacacaggtttctgaaaaccctcattccgaatatgggctctcagagaatgtcgagcgaatagtg
gaaaatgagaagaccagcacggaaaaaatctccaaacagaaagttgatctgcagggcttacctacaagagcatatcttgaccaaacagtagttcctatac
ttttacaaggcctttcagtgcttgcaaaggaaagaccaccaaacccaatagaataccttgcagcttatctcttaaagaacaagacacagtttgaggaaag
aagctagaatggatacatcagcctcctgcattttacataaatattgattattaaagagattgcatttcgtccacagtaacttaaatttaccaattcgtgt
ttccagcaaaagtgaaaattccttttagactgtcacattggggtgaaaagcaagacaaagggaaagggttaatttcttgcaaatttattgtttttgacca
gatttccggttttgtttgttttttttaaataaaaaattgtgacttgcaaaatctttacaaaaattgaaaaaaaaaaaaaaaaaaggatccccctagtttc
ttagaagccggcccggcccacta
3' EST: CF784413 [+]tttttttttttttttcatttttttaagatttgcagtcacatttttatttaaaaaaacaaacaaaacagaaatctggtcaaaaacaataaatttgcaagaa
attaaccccttccctttgtcttgcttttcaccccaatgtgacagtctaaatggaattttcacttttgctggaaacacgaattggtaaatttaagttactg
tggacgaaatgcaatctctttaataatcaatatttatgtaaaatgcaggaggctgatgtatccattctagcttctttcctcaaactgtgtcttgttcttt
aagagataagctgcaaggtattctattgggtttggtggtctttcctttgcaagcactgaaaggccttgtaaaagtataggaactactgtttggtcaagat
atgctcttgtaggtaagccctgcagatcaactttctgtttggagattttttccgtgctggtcttctcattttccactattcgctcgacattctctgagag
cccatattcggaatgagggttttcagaaacctgtgtttgcccctccatgctttgctctgtatccatggtaacactgatttaaaacaggaagcgttcgcac
ctcccgcaaactgtttactgcaggttcctagcctcctctcttacagaatatcccaacgccacaagtctcatcacgcgagactatacgtaacgcagtgacg
catcacgtagccgccttgtgggcgtggccacgcgtgtctaacacggagtgggggggggggggggggaattaattaacttgagggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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