XB-CLONE-4352177
Clone Name: IMAGE:7027301
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Gene:
trappc6b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784418 [+]
taattaatcccccccccccccggctttgctgtcagagatttgccctggaaccaatagtaatttgctgtttttttttccaccccgaatcttcgcccaatca
gagatagcgattgctgtaggcatgaacaagcaggatatggctgatgaagccctctttttgcttctgcacaatgaggttatatcctgtgtgtataaagccg
gcgttgaccagggggatgcggaaaatggaaagtgtattactaaactggagaacatgggatttcgtgttggacaaggtttaatagagcgatttaccaagga
cacagccaggttcaaagatgaactagatataatgaaatttgtctgcaaagatttttggacaacggtgtttaagaagcaaattgataatctccggaccaat
catcagggcatttatgtgcttcaggataataaattccgacttattacccagatgtctgcaggaaaacagtatctggaacatgcaccaaagtacctggcat
tttcttgtggtttaatcagaggaggtttgtctaacttgggaataaagagtattgtcacagcagaagtctcagtcatgcctgcatgcaaattccaggtgat
gattcaaaagctgtagcacaggagtgaagtctgagggggcagtgtttaaaaacgaaaatggattgtaccaaatcactgggtggtcatacattaaaggagg
ccggtccggaaaattaaagggattttaatttttaatttggggagggtccccaccctaaactggaacctttccccattttatttttatggttaaaagggtt
tcccttggttaacacctttttgggttataagaacactc
3' EST: CF784417 [+]tttttttttttttttattaaaaattgtgagtctactcttaactttattaagtatacaaaatatggcattctaagccatattcattttttagggtttagtt
ctcttttaaatgtcccccaatcccgtattttactcaacctactgtaggaatactttctgccaaactatatatatatgctgctttcttatacaaattcatt
caactcccctttttaaattacagaccaataccaataataaaaacattaactgcaattgtccctatatagataggaaagtcctaaagatatttatttacca
gaatgttggcaagggttttcatgggttggggcatgtctgggcataactgggaatagttggttgttacttaatttgtcccagtttcttattttagaatggt
ggtatttattagaataggtttgaaattcagtttactttttttagtggaaataattttaattgtattagtaaatttatatctgtcttcagccaatagctgc
ttacattttacttaataaacatatattctaaggtattttacaatgtttaaaagagatctgttcctaacatgtctagaggcataactcccctgttaaccag
ttatgtacacttccaagaaaaccaagctggtcatacacaatccaatattggcagttagtctgcctatgacacggatctgaggctagctgggccctactgc
tgtatggccaaactgctcagagatctagctgttctggctgcangtcatatagccataatctgctcatggtgagctggcataatgtcagttttagtatatc
catttttcctgtagaaacttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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