XB-CLONE-4352174
Clone Name: IMAGE:7027300
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Gene:
ssr4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784420 [+]
taattaatcccccccccccccggtgtctcagtcggagccatggcaagggtgcagtgtgtgctgctggcggtattgctggccctggcggcctgttctgcgg
aaacctgcacagagcctgttatagtcccttcctattacactacctcagatgcagtgatctcttcagaagtggttttcattgtggagatttccctgtcttg
cactaatggtgctcagaatgttgctctttacgctgatgtgaatgggaaacagttccctgtgaccagaggacaggatgttgggcgctaccaggtctcatgg
agcctggagcacaaaaatgcccgctctggaacatacgaggtgaagtttttcgatgaggagtcctacattctgctaaggaatgttcccactaacaatcagg
atatctcctttttcaagcttcctcctttatgtctatgccgattttcacgggtgcatgggaaccggtccttttgggcatcccccagcaagtaactgggctc
gaattttgatacggccttatttgtgtcatattttataccggccattatttattggccactgtattttttccaaccccacgcccttttaaaccataaaaca
aaacaaggtggtttggggcggccaagtgggatttacccaattgtgtgataccctgccgggatttgtgtacactcgccatatttacggttgtcactggttg
tggccctttgtgtattttgtaccccaaatcatctatctcattttttcaccaccccataaccttggttccacttaacttcttaccatttgtgtttgtttct
ttaacccatttccgcgaggcgttactactctctagttaaataacagatttacagtaatatatatctcttattctcaacgcagtatttgacctgttataaa
tgatccctcaaacgcgcatgttcttcttgtacaacccatcttttttttattatgtcccgcgccctcccccttttttttcatcccccacccccgttatata
ttattgtgaactcttttcttacgccaccnatgagatataacaattgttttacactctcgcgactatcattatt
3' EST: CF784419 [+]aaacacatggcaaaacaaaaagcaattacagaagaactgaaaagcctcatttaaagggcagaaaagatagaataacttatgcttctagattctgtatttc
tatggaaaccaggatgtaagagacaggatgtaggcagtgtgtgtgtagaaaattttattacaagcacaagacaaatctgtacaccgagacccatcgttcc
cttccccaaccctcttctctggttaagcctggatgctgctctttgcagtatatgctgtataatataccaatatgcctatcaatcctgccagtacttctgt
ggatacccaaggaccgttccatgcacccctatgatcaacattgacagtaaagagaggcttgatagaggagatatcctcattgtttctctgagccttcctt
agcagactgtaggactcctcatcgaaaaacttcacctcgtatgttccagagcgggcatttttgtgctccaggctccatgagacctggtagcgcccaacat
cctgtcctctggtcacagggaactgtttcccattcacatcagcgtaaagagcaacattctgagcaccattagtgcaagacagggaaatctccacaatgaa
aaccacttctgaagagatcactgcatctgaagtagtgtaatangaagggactataacaggctctgtgcaggttttcgcagaacaggccgccagggccagc
aataccgccagcagcacacactgcacccttgccatggctccgactgagacacggggggggggggggggaattaattaactcg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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