XB-CLONE-4352160
Clone Name: IMAGE:7027295
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Gene:
snrpd2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077664 [+]
GAGTGGTCCTTCAAAGGAAGCGTTCTACACGCCGGGAAAACGTGAGAAGGGAAAGGAAAGACAAAAAGAAAGATGAGTCTCCTTAACAAGCCCAAAAGTG
AGATGACACCTGAAGAGCTACAAAAGCGAGAAGAGGAGGAGTTCAACACAGGGCCACTGTCTGTGCTTACTCAGTCTGTGAAGAACAACACACAAGTTCT
GATCAACTGCAGGAATAACAAAAAGTTGCTAGGCAGAGTAAAAGCATTTGACAGACACTGTAACATGGTACTGGAAAATGTGAAAGAAATGTGGACTGAG
GTCCCTAAGAGTGGCAAGGGAAAAAAGAAGTCCAAACCAGTGAACAAAGACAGATATATTTCCAAGATGTTCCTTCGTGGAGACAGTGTCATCGTTGTTC
TGCGGAATCCACTGCTTGCTGGAAAGTGATAATGTGTAGCCTCTTTCTAAATTTCCTTGTTCTGGACAAATTCCAACCCTGGCATCAAATAGTTTTAATT
TCTTTACTGAGGATCAGCAAATGTCGTTTTTTTTTTTTTTTTTTTTTTCCTGTCCCCTTCCCCCCCCCCCGCCAAAAAATACTTGTAGACTGTCACAAAG
GTTGTTCTTTAACACCTTTTGTCCCTTCCACCATTATTTTTTTTCTAATAAAGATAATTTGTTAAAACAAAAAAAAAAAAAAAA
5' EST: CF784429 [+]taattaatcccccccccccccgagtggtccttcaaaggaagcgttctacacgccgggaaaacgtgagaagggaaaggaaagacaaaaagaaagatgagtc
tccttaacaagcccaaaagtgagatgacacctgaagagctacaaaagcgagaagaggaggagttcaacacagggccactgtctgtgcttactcagtctgt
gaagaacaacacacaagttctgatcaactgcaggaataacaaaaagttgctaggcagagtaaaagcatttgacagacactgtaacatggtactggaaaat
gtgaaagaaatgtggactgaggtccctaagagtggcaagggaaaaaagaagtccaaaccagtgaacaaagacagatatatttccaagatgttccttcgtg
gagacagtgtcatcgttgttctgcggaatccactgcttgctggaaagtgataatgtgtagcctctttctaaatttccttgttctggacaaattccaaccc
tggcatcaaatagttttaatttctttactgaggatcagcaaatgtcgttn
3' EST: CF784428 [+]tttttttttttttttgttttaacaaattatctttattagaaaaaaaataatggtggaagggacaaaaggtgttaaagaacaacctttgtgacagtctaca
agtattttttggcgggggggggggaaggggacaggaaaaaaaaaaaaaaaaaaaaaacgacatttgctgatcctcagtaaagaaattaaaactatttgat
gccagggttggaatttgtccagaacaaggaaatttagaaagaggctacacattatcactttccagcaagcagtggattccgcaaaacaacgatgacactg
tctccacaaaggaacatcttggaaatatatctgtctttgttcactggtttggacttcttttttcccttgccactcttagggacctcagtccacatttctt
tcacattttccagtaccatgttacagtgtctgtcaaatgcttttactctgcctagcaactttttgttattcctgcagttgatcagaacttgtgtgttgtt
cttcacagactgagtaagcacagacagtggccctgtgttgaactcctcctcttctcgcttttgtagctcttcaggtgtcatctcacttttgggcttgtta
aggagactcatctttctttttgtctttcctttcccttctcacgttttcccggcgtgtagaacgcttcctttgaaggaccactcgggggggggggggggat
taaattac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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