XB-CLONE-4352149
Clone Name: IMAGE:7027290
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Gene:
rpl32
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077707 [+]
CTCTCTTCCTCGGGCTGTCTGCGGCGAAGGCGGACATCTCGAACCGGTACCATGGCGGCCCTAAGACCTCTCACCAAGCCCAAGATAGTGAAGAAGCGGA
CAAAAAAGTTCATCCGCCACCAGTCAGATCGCTATGTTAAGATTAAGCGTAACTGGCGCAAGCCCAGAGGTATTGATAACAGAGCCCGCAGAAGGTTTAA
GGGACAGATCCTGATGCCCAACATCGGTTATGGTAGCAACAAAAAAACAAAACACATGTTGCCCACAGGATTTAAGAAGTTTCTGGTGCACAATGTTAAA
GAATTGGAGGTTCTTTTGATGAGCAACAAGACTTTCTGCGCAGAAATTGCCCACAATGTCTCCTCTAAAAACCGTAAGACCATTGTGGAAAGAGCAGCAC
AGCTTGCCATCAAAGTGACAAACCCCAACGCCAGACTTCGCAGCGAGGAAAACGAATAAACTTCCTGTCTCTGATGTTGCTCTTTGTAATAAATGAAAAA
GTAAAGCTAAAAAAAAAAAAAAA
5' EST: CF784435 [+]taattaatccccccccccccgctctcttcctcgggctgtctgcggcgaaggcggacatctcgaaccggtaccatggcggccctaagacctctcaccaagc
ccaagatagtgaagaagcggacaaaaaagttcatccgccaccagtcagatcgctatgttaagattaagcgtaactggcgcaagcccagaggtattgataa
cagagcccgcagaaggtttaagggacagatcctgatgcccaacatcggttatggtagcaacaaaaaaacaaaacacatgttgcccacaggatttaagaag
tttctggtgcacaatgttaaagaattggaggttcttttgatgagcaacaagactttctgcgcagaaattgcccacaatgtctcctctaaaaaccgtaaga
ccattgtggaaagagcagcacagcttgccatcaaagtgacaaaccccaacgccagacttcgcagcgaggaaaacgaataaacttcctgtctctgatgttg
ctctttgtaataaatgaaaaagtaaagctaaaaaaaaaaaaaaag
3' EST: CF784436 [+]ttttttttttttttagctttactttttcatttattacaaagagcaacatcagagacaggaagtttattcgttttcctcgctgcgaagtctggcgttgggg
tttgtcactttgatggcaagctgtgctgctctttccacaatggtcttacggtttttagaggagacattgtgggcaatttctgcgcagaaagtcttgttgc
tcatcaaaagaacctccaattctttaacattgtgcaccagaaacttcttaaatcctgtgggcaacatgtgttttgtttttttgttgctaccataaccgat
gttgggcatcaggatctgtcccttaaaccttctgcgggctctgttatcaatacctctgggcttgcgccagttacgcttaatcttaacatagcgatctgac
tggtggcggatgaacttttttgtccgcttcttcactatcttgggcttggtgaaaggtcttagggccgccatggtaccggttcgagatgtccgccttcgcc
gcagacagcccgaggaagagagcggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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