XB-CLONE-4352146
Clone Name: IMAGE:7027289
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784438 [+]
taattaatccccccccccccccgactagtctttgtgagtactccatctgcaggagggctcgcacatagaaaaaacaaatatatatataaataagtgcaag
caagccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcacacaagccct
cagtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcggctaaacca
gccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaagttccagcaaagggtaaaaagg
gagcaaagggcaaacaggacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggagaaacaaaaagtgacgaggcccctgctt
ctgatggaggcgacaaggaatcaaagtctgaataaccttcatcttgtaccgagtcttttatcagtggttcctgtacctcccttgtacaattcagaggaat
atttttatcaactattttgtaaatgcaagttttttaagtagttttaattctagaaaaacacattttttaaaataggaggggaatcccccaccattttttt
gttgtaaaaaaatttttttttaaaacccttatgaatttggtacattgccccctgttatggttttgtaaaggcctatggaataccattaccagtt
3' EST: CF784437 [+]tttttttttttttttctttttcaaccaggttttattggtttggtgatcagtacacagacactatgaacaatctgtacattttttttttcctttttaaaac
tctagtagattggaaatctaggaaaaatatgaaaaaagaaaaaaaataaaaacaatgtcgttgaagtcattggaatccattttctagcggctttccggtt
tttttttttttttttaaagttcaaagagaagttgtttcgtaaaaaatctagcacccctcgtaccagcatctttgatgcaaataattcaacccatttctcg
gccaagaaggagcgaacacgcataggtgccaccttggcaggaaagagatgcaaacacatgaaccgccgtgtcctttttgtttttctccctacttcacatc
acactcactacacactcaaagaagtttccccagaagagattcacagcacttttacaaagttactgaaaataaaataccactacccagaaaaaaaaaaata
aaatggtaacatcacagatagtacacaattctttagagggaaatccgtcaccttgggagatttattagtttaaaagtgtaaattgtacgcaggacaacat
ggagtacaaagaacagttaaaacgttaagacattttaataaccgtgatcttttgcgccagttgtaccagtatcggggcactggcctgttcgaccgggcag
cctgcataaaagaactaagccgtttttggaaacaaaataaaaaaaaaacattttttaaaactggttcaagggcgtttgcccctcccccaacaaataaaac
tggggttgtggggggggtaagaaatataaacggggggaaaagaaattttccaaagccccttttaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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