XB-CLONE-4352141
Clone Name: IMAGE:7027287
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Gene:
banf1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784441
[+]
taattaatcccccccccccccgggcgggaagacgtgaaaagagtgaaaatgtcatcaacatcacagaagcacagagactttgtggcagagccaatgggag
aaaagccggtccaatgtctggcgggtattggcgaaacactggggcataggcttgaagaaaaagggtttgataaggcttatgtcgtgttgggtcagtttct
cgttttgaagaatgatgaagagcttttcaaggattggctgaaggatacttgttccgccaatgccaagcagtctagagattgctttggctgcctaaaagaa
tggtgcgatgcctttttgtaaagtagtatctctcctctttgtgatgcctttgcaaagccagctccagagagaacccccaagtcacagctaaagccacaga
aatttgccagatgcatcattatcctgaaggccaagaaacttgcccacttttgagagaattccattggactaaaaaaaagaaacctatgggcatatggaga
attttaaaaataacaaaacagacaatccatgattaaccacaaataaaataatccacatattgatcccacaagatgattcgtttttgatgcataaatagcc
acggcccgcaatatttgctaatgggtacgtttaggttttatggggaatttcagggggcatggtttttaatttattttaaattacactaaaaatgcaaacc
gggtcctggtttttaacctttaaacatctggttttcctttcccctattggatttaataaaaataggaaaattgccttttgggaaattctgggccc
3' EST: CF784440
[+]tttttttttttttttctttttaacagatgcaaaaatttatttttgaaaaaaaaaaaaaaaaacaaagcctgaaaatacttggacaaatggacaggtataa
aggaagtatttcaacatgaaagtatttgagaattacataaaaaacaacatgagaaaccagcgcactgctgcatagcctgtaacagtggttgccaaaccca
caatattatctgaataattccctagtaaattcttacagatactactaagcagtaacactgtacatccacatgcaacaattgtacaaatgtctatgcactt
aagagctactgaggactacagcatttcctgcagctgggtaggaacacagtgagcgaggtagcaatggttttgctacaacctatataataagggccagatc
tcacaaagcaatttcctattttatataatcatatagggaaggaaagcagatgttaaagataaaaacagaacatgttgcattttagtgtatttaaaataaa
taaaaacatgccactgaaattcccataaaactaaacgtacacaatagcaaatattgcggccgtggctattatgcatcaaaacgaatcatcttgtgggatc
aatatgtggattattttatttgtggttaatcatggattgtctgttttgttatttttaaaattcttcaaatgcccatagggtttcttttttttagtccaat
ggaattctctcaaaaagtgggcaagtttccttggcccttcaggaataatgaatgcatcctgggcaaatttcctgtgggcttttagcctgggacactgggg
gggttcctctccggggaactgggcttttgccaaaaggccttccccaaaaagaagga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: