XB-CLONE-4352128
Clone Name: IMAGE:7027282
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Gene:
sec61a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784448 [+]
taattaatccccccccccccgagtctaaggagagaccgcggcgcaacaggaccggtcccccagcccgtcacacccctagaggccactgagcccaccgcaa
caccttcaccatgggcattaaatttcttgaagtcatcaagccattctgtgcaatactaccagaaatccaaaagccagaaagaaagatccaatttaaggaa
aaagtactatggactgccatcactttgttcatcttgcttagtatgttgccagatcccactctttggtattatgtcctcggattccgctgatcctttctac
tggatgagagtcattttggcttccaacagaggtacccttatggagctcggtatcactcctattgtcacctctggtctaatcaagcaacttctagctggtg
ccaagattattgaggttggagacactcctaaggaccgggctctcttcaatggtgctcagaaattatttggaatgatcatcaccatcggtcaagccattgt
gtatgtcatgactggaatgtatggggatccttcgcagatgggggctggcatctgtcttcttatcccatacagctctttgttgctggcctgattgtcttgc
tcctggatgaattgccacccgaaggctaaggactgggcctcgggaaccacacgtcaatgccacaaacatccgccaaaccattggtatgagaaagtattca
agtcccacttcggggaaacacgggccgaaggccttgaattaaaggggacaaacctttggccctggtttccctttgttt
3' EST: CF784449 [+]gcatgaagactatgtaaaccactgcatgaaccggatcgtccagtaccgaaccaaatgattccggcggtgagaggtaataacataacccgcctacaggata
ggcccgagcaggacctccagtcgttgcatcagaccatgtgcccaataagttgacaaggaggttgccactgaaacgggcagacagcatctgggagataacg
tacagatttgagaccagggcagactggagaatgatggggatgttagaagtatagaaaagcttgattgggtaagtgttgtactggccacggtaccgggctg
acttgattggaagatccactctaaagccctggaagtatatgactactgcgaaaacgaaaatggtagcgatgaggttcatgagatttggcaggttctggcg
atagaaggcctccctcagagcgcggactttgtcagtacgtgtggcaagcaagtgaaacagggcaatgattgctccctcaaactcagtgcctctgcctgtg
ttcacagtagttggactgaataccttccatacaatggtttcgcagatgtttgtagcaatgaagagagataccagagcccagaccatagcccttcctgtag
caattcatccaggagcaagacaatcaggccagcaacaaaagagctggatggtggataagaagacagatgcccgcccccatctctgaaagatccccctaca
tttcaagtcatgacatacaccatgggcttgaccgaatggtgaagaatccatttcaaaaaaatttctggagcac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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