XB-CLONE-4352120
Clone Name: IMAGE:7027279
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Gene:
fkbp8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784454 [+]
taattaatcccccccccccccagggagcaggatttggtagcgaattagaaagctgcttacagtttggtctaggaagtgctttcagcctgcaggtcacact
cttttcttgccaagaactagagatgacctctgcagaagaatcaatctctgatacaattgatattcatgaacaaggagaacaaacaattgaaatccatcca
ggtgagaggactgagccaaacccagctcttctggagagcacagaggattttgaaataatagacagcgcagtggaagaagatgatctgagcgaccttccac
ttctggaggatgtagataagaaggagaagcctgcagcagacaatgatttaaatacacaacagacagttgcaaaagaggaggaagaatggctggatgttct
angtaatgggatcttgaaaaaaagattttaatctctggaaagggtgctgacagtagaccaagaaaggacaggatgttaccgtggggggctaagtccatgc
tgggagatggaaatgtantanaagaacaagaaactcttaccctttacactgggagatgggagatgtcattcaggccctgggacctgggtgtttcggctca
tggaagcaagaagaaacttccgctccatgcatcagatgcccaagtactgtttatggaaaagaaaggcagggatttccaaaattttccccccaactcccaa
cttgaaaccctgaaaatggcccttggctgggatggttccgggatgggaccccaattctgggaaaaaacctttccgggga
3' EST: CF784453 [+]tttttttttttttttattttttttttaaactgaaagttccagtttttaatgaaaaaacagtgtgaaacacaccaacaacttaaacaactagtatagcaga
tcttggttgtactgctctaacacgacataatacaaagttggtcctctatgccttccccctgtacatgatagagagctctccaaactttacctttctctag
aattatatatacatctatagttacacataccatatcatactttgagatgtttacatgtgtcctagaagtttctgcttaaaataattataactgatcagta
cctcacaagaaatatgtgagatacacatgtattacacacatgtaatatgtactttcatttaacaacaatatctatgtgccgtgtcatcatgacatcaaat
tatttctgtggttacaggtcacccagattcatttaatctccctctcttgcactggaattcaaaggccaggaatgaaaaggtttctgcagtctttggcttc
aaaagcttgctggacatatacctcctaacatatatatctatgtatctatctcagatagagcgccattgtgccactgactctattctaagggtttgaattg
aatttcttagtgttaccaatgccaaatgaaaacgccccttttttaaattacacatttccctgaacaggtgcacaattttacatttatttatgtgagcaaa
attggagttcacctatataaaaaggtacagtttatgcatattttctcttttctcacttggacactgtttccataacaaggtttcacctccaggggttgtt
ttgagaatgggtggacactgta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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