XB-CLONE-4352111
Clone Name: IMAGE:7027276
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Gene:
sh3kbp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784459
[+]
taattaatcccccccccccccggctgacttcttggacattttagactgcagacacttttcacaatgggagaagaggcaatgggaaagaaaatgccaccca
ctgtgacttcacaggagccgatgaaaatagagacagagagcaaaacaaaacccagggagtactgtaaggtcctattcccatatgaagctatgaatgaaga
tgaactgacaatcagagacggtgatattgttactgtattaagcaaggaatgtgttgatgccggctggtgggaaggagaacttaatgggaaaaagggtgtt
tttccagacaactttgtcaagctcctcccttctgactttgaaaaggaactccatagaccgaagaagcctcctcctccatcagctcctgtcaccaaatcag
gcacaagtggtacctcagagagaaaacaggacatcaggaagatccccccagagagaccagacaccctgccgcagaggccagatgagaaggaaaggagtga
tagagaccacaaagtgtcagactcactgaagcactcagtacccgctatacctccaaagaaccctcgtcctcctaaacaaactctttaagtaaacctggga
cactgcctcctcgaagaccagaaaaaccagttgggcccgtaaaccccccggaaaggattgaaattccaaaatcagaagggggggaatcaataacagtggc
ccaataccccctgaaccgaaaccccctcccgaataaaagcattggacttcggatggtaaaaaggtttttgaattccatttgtgaaaaatggg
3' EST: CF784460
[+]tttttttttttttttgaaatacattttattcatggaaaaaatatcatcagtaaaaagtaccactctgaagtgcactgcctgagtttattcatttgcagtt
ttctgtctccccggagcgccagagaggcgaggagcaataccatgtagttccctctggcacccgagcagcggcggctaagtaggagcacttcagcacgagc
tggaaataacgtcaacttgtcacatgtgccacattataaatatcacaaaggagtctctgataggtggagctgctgttgtgctttccgcttgtttgaggca
aatctgaattcatttggcaccaggttctgttggctgatggggtcggaagtgttcggtcattcgaggacttgcagcggattccatgacaccttctctttgt
tctttgccttatttggattgtagagctttcttgatttcgttgacgtccagctgtaatcttaatcgtatctttttctcttcgtctagttcaaaaagtaatt
gtttgatctccttcttctgttggtctttcattgtttcaacgatagatctcagctcccttgactggtgactgtaactcttctaagggcgactgactatgat
cagcccttctgtcttggtttcggcccctcgaaaaaggtggaagagtttcgacctgggggctgccaacggcaaacggggtggaaaaggctgaggggggctc
cccgatgcccccatgaaaaaaaagcttcacctttggaaaaattagagccctcccgggccttggggggggaaaaaacaatttcctgttttctcgggggcat
tttggggagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: