XB-CLONE-4352074
Clone Name: IMAGE:7027263
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Gene:
senp6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784484 [+]
tactctcgtagtgttttgaccacagtggaacgagaaggacctttcagagaatccatcaaaagtatacatggctgcttgcagaagaattgcttccgatgcc
agtttccagcttctgaagtataattttcatctgcaagccctgcgtcatcactgcattcatccgtattatcctgatcatcagagaaatctatgaattcatc
ttcagatttcttagtatcatcttctgtctctctatagcttatctgaattctgtgcaaaccatctgttggttttactggtgttttacactcatttgttgtt
ccatttatatcatcagtgcatgtttcagagtctgaaaatacagatttcatagaaatgtctacttcttgatgcccttgatcactttttcttgaccgatgcc
ttgttaagctgtgactacgcttagctcccaaagtatcaatagcatctgtcccagtgctgagtgttttctttaggggtgatttagaagaaccaccttctaa
agtagtctctgaagtattctgaggaagagaagtactggtgttatctccttctccactggagccggggaagaaacttcttgatagtaaggattggggtaat
gctcaggcttttctaatccagggaaaacaatgacagccaaaaaccaatgagcagcttcgttaagcggaacaaatatgaaatccttttgaacaatgtcaac
atgtcgagtccatgtttttcactcttccatgttcttctcttggggtaatgttaaggtttggagtaacctggaagggtttccttcctttcccccttggata
aagtccttttaaacgaaaaaaaaaaattgan
3' EST: CF784483 [+]gctcctcctccccccatcttctcccggttggggcctgtttgttttctaagcttcgtgctgacttggttttctttggccttaattttacgtcacaggcacc
gggctggtattcacccctaccctgcctccttccccgctgccctgccgcctcacggctatggagtgagctgaaagatggcgggcggcggagggagccagct
tctggaagccttggatagatctcagtcaaagaaagatggagggtttagtaattgcggtttttacaactctgaagacagcgaagatgataccgaaaaagat
gaagcaaatctgctgagtttagatgaaagtgatgatgtcgacttacaaattacagaacaaaagcccaaacaccatagaactggaacagccagagctcttg
gagagactattagaacatatgaaagaagaggcagaacattgcacttgagagctttgaaaggcaatgctattggattaaacatgttgggaaccagtaaaaa
gcttggggaaaatgctcaaaatataccagtctctagtggaacaatggtacacggcagaatcttccatcatgcaaatattccaaggtctactgtcaagact
gcggcacaaaggaaggaatacccagcccatgtgcaaaaggttgaatctgatccaggaagagtacatacttctccaagatcagaaaacatgcaggaaaaga
aaagaagaacctgactttgaatcatctgaacccgagaagataaagagaaaggttcaacagagacgacgtagtagtatacaagaatccgaatatcttcttt
ttcttcacacttcccaaaatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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