XB-CLONE-4352068
Clone Name: IMAGE:7027261
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Gene:
rbbp7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784488 [+]
taattaatcccccccccccccccccggtgagtgtttggcgcgggaagctgcagcaggaccgcacaagcgcggaacactgggtttttagtcaccgcgaggc
cggcggcccttgcatctgcacaggagtagagaagatggcgaataaagagatgtttgaagatacagtggaagaacgtgtcatcaatgaagagtataagata
tggaaaaaaaataccccatttctgtatgacctggttatgacccatgcacttgaatggccaagtctaacagttcagtggctcccggatgtgactagaccag
aaggaaaggactatgcgctgcactggttggtcttaggtactcacacttcagatgaacaaaatcaccttgtagtggctcgtgtccagattcccaatgacga
tgcccagtttgatgcctcacattatgatagtgagaaaggagagtttggtggctttggttctgttagtggcaaaattgaaacagaaattaaaattaaccat
gaaggagaggtgaaccgcgctcgttacatgccccaaaatccatgtatcattgcaactaaaactccttctgcagatgtgctggtttttgattataccaaac
atccttctaaacctgaccctagtggagattgcagtcctgatctcagacttagaggacaccaaaaggaaggctatggcctatcttggaaatcaaaccttag
tggacaccctctcagtgcatcagaagataaaaccgtttgtttatggggacataaacccccggaaccaaaaaaagggcaagggtaattgaatgccaaaaac
catcctttacctgggcccgt
3' EST: CF784487 [+]ttttttttttttttattccatcagccgcttcagcgagtcccccaggtatccaatacaaagagcaagaatattacaatacaatttacaaagaaaatgtcaa
tacatgtactcagaattgcaaattaacttacacaaacattaaccacgtcacaaatgtggcagcatctgcaatagtgagtgtgaataagaaaaaggaaaaa
gatctatgatccttgagcttctagctcagaagctggtatgtcaggttcttcatcattgtaaatattttcagccatctgccatatttgcatgatgttatct
tcagacactgaacatatcacccatggttcatttgggttccagctgaagtcagagatttttgctgtgtgccctccatgtataaacaaaagctcaggaggtc
catcctcagcatcttctgctgactgctcttctccaattttacttaaatcccagacattaagtcttcgatctgtaccgctggaagctaaaatagtttcatt
atgaggagaccagtgaacctggaaaatttcatccttatgagattcgaaggaatggagcttcagctttaaattcctgagatcccaaagagctacagtctta
tccgcagaacctgtggccaaaatgaactcgctgtatggattgaaggacaagcaattgacctcagcagtatgcgcatccactgaatggctggggtttagaa
gtggtatttgaacttgtgtcccagatcataagcttctgatcatctgcaacagagccaaataaaggattcgtgcagaaaggtgccacgccaacaatcctct
actactggcgaaatgggcaagtaaagaatggctttttgcatcaacta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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