XB-CLONE-4352059
Clone Name: IMAGE:7027257
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Gene:
eif4a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784494 [+]
taattaatcccccccccccccgagcttccgctcagaagaatgtctgcgagctacgagagtcgacccagagacaatggacccgaaggcatggagcccgatg
gggtcattgagagcaattggaatgaagtcgttgatagttttgatgacatgagcctatcagagtctctgcttaggggtatttacgcctatggctttgagaa
accctcagccatccagcagcgagctattatgccttgtatcaagggttatgatgtcattgctcaagcacagtctggaactgggaaaactgccacctttgcc
atttccattcttcaacagatagaactggatatgaaagccacacaggccctggtgttggcaccaacccgtgagctggcccagcagatccagaaagtagtta
tggccctcggagattacatgggtgcctcttgccacgcctgcattgggggcaccaacgtaagggccgaagtacagaaactgcagtcagaagcccctcatat
agttgtgggcacccccggcagagtgttccgacatgttaaacaggcgttacctgtcccctaaatacataaagacgttcgggttggatgaacccgactagat
gtttgatagaggtttcaaggatcaattctcccacatttccccaaagctgaccactatagccccaggtggtttttggctctccgggcaaccatgccccgcc
caaacttgcttcgaaggtggacccaaaaaagtttcatgcgggggaacccccattcttgtgatccccgggtgtttaataaggggcggaaaccctctcgccc
tcccggtaggggtttatttaaggggcaagtttctataatcttccacctttttggaaaattgtaacgcgggtagctgggaaaaacgcct
3' EST: CF784493 [+]gatcggaactccctcataatgacgtctctctccttctggtccatgtcgccgtgcagggcggagacagtgaagtccctcgcgtgcatcttttccgtcagcc
aatccaccttccggcgcgtgtttatgaagattacggcctgcgtgatggtcagtgtctcgtacaggtcacacaatgtgtccagcttccactcctctcgctc
cacgttgatgtagaactgcctaataccctccagggtcagctcctcctttttaaccaggatccggatggggtcccgcatgaacttcttggtcacctcgagc
acgtcggcgggcatggtggccgagagcaaaaccacctgggcattactgctcagcttctggaatatgtcgtagatttgatccttgaaacctctactcaaca
tctcgtcggcttcatccaacacgaacatctttatgtatttaggggacaggtaacgcctgtttaacatgtcgaacactctgccgggggtgcccacaactat
atgaggggcttctgactgcagcttctgtacttcggcccttacgttggtgcccccaatgcaggcgtggcaagaggcacccatgtaatctccgagggccata
actactttctggatctgctgggccagctcacgggttggtgccaacaccagggcctgtgtggctttcatatccagttctatctgttgaagaatggaaatgg
caaaggtggcagttttcccagttccagactgtgcttgagcaatgacatcataacccttgatacaaagcataatagctcgctgctggatggctgaaggntt
tctcaaagccataggcgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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