XB-CLONE-4352056
Clone Name: IMAGE:7027256
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Gene:
h3-3b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784496 [+]
taattaatcccccccccccccacgttagtttgtctcctcgtttgaccgtttgggttacgctcccgggagtgatcgaataacaagaagcaatggctcgtac
aaagcaaactgcccgtaaatccactggtggaaaggctccaaggaagcagctggctacaaaggctgccaggaaaagtgctccttccacaggtggtgtgaag
aagccacacagatacaggcctggcactgttgccttgagagaaatcagaagatatcagaagtctactgagctgttgatccgaaaactaccattccagcgac
ttgtaagggagattgcccaagacttcaagactgatttgaggttccagagtgcagctattggtgctcttcaggaagcaagtgaggcttacctggttggcct
ctttgaagataccaacctgtgtgccattcatgccaagagagtaacaattatgcccaaggacatccagttagcaagaagaatccgtggcgagcgtgcttaa
acattttgctaggaacttttttttgaacttagtttttttttttaccattgtacatacaagtgtgatgtcttttattttacaaagggtttgttaactgtag
agcagttgaaagcaacatggtagtaaacattttatatgacattcctcttaaacctcaaggtttttcaaaaaatttgtattctgcagctaattcttttttg
gaccctccccctttaaaacaataatgcatggcccaatactttcatttcttgtaaaataaattttgagtaaccctttttctggggccaccctccccttttg
gtaaataaaaccccctttttcctactaatntgttcgaaagaaaacaaaaaaaaa
3' EST: CF784495 [+]tttttttttttttttttttttttttttttgagggagtggaaagtttatttacaatggagttacacagaaagatactaaattatgtaaagaatgaagtatg
ggcatgcactatgttttaaaggagagggtcacaaaagactagctgcagatacaaatttctgaaaaacctgaggattaagggaatgtcatataaaatgttt
actaccatcttactgtcaactactctacagttaacaaaccctttataaaataaaagacatcacacttgtatgtacaattgtaaaaaaaaaaactaagttc
aaaaaaaagttcctagcaaaatgtttaagcacgctcgccacggattcttcttgctaactggatgtccttgggcataattgttactctcttggcatgaatg
gcacacaggttggtatcttcaaagaggccaaccaggtaagcctcacttgcttcctgaagagcaccaatagctgcactctggaacctcaaatcagtcttga
agtcttgggcaatctcccttacaagtcgctggaatggtagttttcggatcaacagctcagtagacttctgatatcttctgatttctctcaaggcaacagt
gccaggcctgtatctgtgtggcttcttcacacaacctgtggaaggagcactttttctggcagcctttgtagccagctgcttccttggagcctttccacca
gtggatttaccggcagtttgcttttgtacaagcattgcttcttgttattcgatcactcccgggagcgttaaccaaacggtcaaacgaggagacaactacg
tggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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