XB-CLONE-4352044
Clone Name: IMAGE:7027252
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784504 [+]
taattaatcccccccccccccgctcttcctcaccgggtctgcggcgagttttaagtgtccaccgccaaacatctaacaatccacaatgggaaaggagaag
actcacatcaacatcgtcgtcattggacacgtagattctggaaagtccacaacaactggacatcttatctacaaatgtggtggtatcgacaagagaacca
tcgaaaagttcgagaaggaagctgctgagatgggcaagggctccttcaagtacgcctgggtcttggacaaactgaaggccgagcgtgaacgtggtatcac
cattgacatctccctgtggaaatttgagaccagcaaatattatgttactatcattgatgctccaggacacagagacttcatcaagaacatgatcactggt
acctctcaggctgactgtgctgtcctgattgttgctgctggtgttggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgccctcc
ttgcctacactctgggagtaaagcaactgatcgttggtgttaacaaaatggattcaactgaacccccatacagcccgaaaaaaatatgaggaaaatcgtt
aaaggaaattcagccacattcctccaagaagaattgggttacaaccccttgaaaccggttggccttttgtaccttattttttgggatgggaaacgggtga
aaaacaatggctttggaagccccagccccccccatattggccctttggtctcaaaaaggggcggggaaaaaactccctttacgttaacaagaaaggggat
ttattggccccccccggaaaaaaataacacccttggcgatgggaaaaagtttctttttgaatctggccattttttttgccccat
3' EST: CF784503 [+]tttttttttttttttatcttttaacttttttttaatggtctcaatttggtgacagattttggtcaagttgcttccatttagaaattactggatttaaaac
actaataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcattgtaatcattaaccagtctttgact
attaaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgagagatgctggaaatattcatttgcctt
tctgtgctttctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactcctacagcaacagtctgcctcatgtcacg
gacagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaacaatggcaacatcaccagacttcagg
aacttgggattgtcttccagtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagcaatgtgagctgtgtggcaatccaaca
caggggcatatccagcaccaatctggcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttccattggtggggtcattcttgctgtcac
cagcaacgttgccacgaccgacgttctttaaaaaaaacgttttttcaggttaaagccaacattgtccaccgggcatggccctcgcctaaaggctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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