XB-CLONE-4352032
Clone Name: IMAGE:7027248
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784511 [+]
taatgaatcccccccccccccccccggcatccttgctctctgctttgtcctagctgacttgtcggagaatatctaccaataaacagccatcatgacttcc
tataggtccagctctgcttcatactattcaagctcatccggcaaaggtggctttggaggcagcagctatggaggcagcagcttggcaggcagcggctttg
gaggaggctttggcagcggtgcaggctctggcttctcatcctctgggggtaactttgccatggcggcggcagccagctccagcttaagcaacttcggtgg
aaatgaaaagcacgccatgcagaacctcaatgaccgccttgcttcctacctggagaaggtcagagccctggaagcctccaatgccgacctggaagggaag
atccgcacttggcacgagaagcaaacaggcaccggccttggagctgggtctaaagactacagcaaatattttgagatcatcagtgagctgaggggcaaga
tccacggtgcaactgttgataacgctacagtgaccccgcaaatcgacaaccccagaatggctgctgacgactttagacttaaatttgaaaaagaacttgc
ttcttccccaaaagggggaggcttaatatcccctggcctgcataaaagtcctggatgaacctgacccttgggcttagaagggaccctggaaaatccataa
ttgaaaaccttgacctgaaaaacctgtgcctacccggaaaaaaaaaaccttgtggaagataaataatgccttgccaagaagccataaaggccta
3' EST: CF784512 [+]tttttttttttttttcgagttaaagatcttttattgctttatgagacatttccataatcatttcttctttcttacgcaggtcttctctcttgtttcaggg
gaaatttctgttcatttgttcctttatctttcccgactgcccttggatttatgacactgttgaagaaacaaccttcccatctaccacctcttcgacaatg
gtttttactgttctggttctggttgttgttgttgatgatgattctgagctggactccagggaacttgcctttgacactgtggtcatctgtcccagttccc
catccagcaggcgcctgtatgtttcaatctccatctccagcctggtcttaatgtcaaggagctgcttgtactccatgttctgatgttccatgtcggacct
gatctgcagaagctgttcctctaggctgctgatagtgttctgaatctgtagaagttgcgctgaatagaagccttgtaattcattgaggttgccttcaact
gattgtttcattgccagctgggattgcagctcaatttccaagctctgaaatgaccgtttcagctcagtgatttcgctcttgctggcctgaacttgttcaa
ccccaactgaaatctctttctttagctcaccgctctttcggggggaaccacaacttttgatcccctgcgggttcttctcaagccggttttttctaagtca
gcctttaatgttatttcaaaaatggtggggcaaaatccaccccctggaagccccgttccattcttcaaaaattcactttttccgggcgctcctggctttt
tgggaatgcaaaaacctccccccaaaagggttcttcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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