XB-CLONE-4352024
Clone Name: IMAGE:7027245
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Gene:
sf3b6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784517
[+]
taattaatcccccccccccccgccggtttgtgcgtgtggtgttggaaagcggattgtttttatcgcgtctgggaaaggtttcttattcggcggcaccatg
gctatgcaagcggccaaacgcgccaatatacgcttaccaccagaagtaaacagaattttatacattcgaaacttgccctataaaattactggggaggaaa
tgtacgatatatttggcaaatatggacccattcgacagatcagggtcgggaacacgccagaaaccaggggcacggcatatgtggtctacgaagacatatt
tgatgccaagaacgcctgtgatcatctctccggtttcaacgtctgtaacagatacctggttgtgttgtattacaacgccaacagggcgttccagaagatg
gacacaaagaagaaagaggagcagctgaaacttctcaaggagaaatatggaatcaacacagatccccccaaataagacttccttctgttttgtttttttt
ttttttcaatttttttagacattgtttttgttcattttttgcaagctttataaatgtttttgaatgacttatggacttctgtcatgtgacctagtcaata
tttcccaggatgcctgagtaccgctacccctccgaagcattgcttagtaaaggagttcccagtggaatgtaatagatcacggtgaccaattattttcatg
ggaatattcctatagtacaatataatatagctgtcccagggggtgagccttcatttcctgaggggctga
3' EST: CF784516
[+]tttttttttttttttttttatcattancaccgggctttagttcccctttatttctggtcagcacattcctccatacacaggattcccatgcacaaagaaa
atgacacatgaccagactaataaatcttgtctgttttaatgattaacttacaaaaaatacttttcccaggaagcgggcctttaaatggtcacggagtata
aatatctggatatttaaactggacgttccactaactctgaagagagagaaatgcgactcaatgcaatgctgcagtttctctgcccgtcgcattcatatgc
cccatgtacaatgcatgcagcccatcaggaatgaatgctcacaccatggacagctatattatattgtactataggatattcccatgaaataattggtcac
gtgatctattacattccactggaactcctttactaagcaatgcttcggaggggtagcggtactcaggcatcctgggaaatattgactaggtcacatgaca
gaagtccataagtcattcaaaaacatttataaagcttgcaaaaaatgaacaaaaacaatgtctaaaaaaattgaaaaaaaaaaaaaacaaaacagaagga
agtcttatttggggggatctgtgttgattccatatttctccttgagaagtttcagctgctcctctttcttctttgtgtccatcttctggaacgccctgtt
ggcgttgtaatacaacacaaccaggtatctgttacagacgttgaaaaccggagagatgatcacagggcgttctttggcatcaaatatgtctttcgtagac
cacatattgccgtgccccctggttttcttggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: