XB-CLONE-4352021
Clone Name: IMAGE:7027244
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784518 [+]
taattaatccccccccccccctcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaac
ccaaaaaaaaaaaaaaggggtcccctttttttaaaggggccccccccccgggggaatttggccggggcccaattttttttttgccccttaaaagggttaa
aaaaaaagcaaaaccctcccaattttccaaaaaaaagcttttttttcccggcattcaaattggggtttggccaaaccccccaagggtttttaacagggcg
gggtggggaattaatttgggccaaccccagggccggaaaaaacccttaaaaaaaaaaattggttagggacctttggaggggaaaaaaaccacctggggaa
agggggtcaattaggggggggaaaatccccaggtcccccaacggggaaaaattttcaaaacatgccttccaattaatccccaacccgggtggaaaaatcc
ccaggccccccacgggggaaaatttttcaaaacaggcctcccaattagcccgcaacccaagtccccccccaaattccccccatccccccccaaaacccgc
ccagttccccccatttccccccccaggggggaaaaaattttttttttttttttaaaaggcccaaagccccctccgccttctgaaattttttccaaaaatt
ttgggagaggcgttttttgggaaaaaaaaaaccccctaaggtttttttaaaaaaagcttttaacaccttgggcaactggccccttctttttaaaaacttt
cgggaactggagaaaaaccctggt
3' EST: CF784519 [+]tttttttttttttttgttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttaagggg
gggggggggataaattaaccccagggcaaaaaaaaaaaatacctttttttccgcccccaaaaaaacccaaggccctttgaccccggaaatgaaccccctt
ggaaaaaaaggcaaaaggaaatttttcccaaagggccccccaaacccaatgggtaaaaaacaaaaggttattatttaattttaccccccttctaaaaaac
ccaagggggggggcccaaaaaccctaaagggggaacttaccccccccttggcccgggaaaacccctttccaaagcccccttccccccccccaaagcggaa
accgcccccggttctcccatggaggccaaaacacccggaaagggctcccccggcaatttaacggttcccaaaaaaaaccccccttaaaaaaacccccccc
cgaaacccccccccccccattttccccaagggggcgaaatttttccaacattttgaaaaacccccttaattttggggccaaaaaaaaccccccttgaccc
caagggggggaaaacttgaaaaccccccggaaacaaaccccttttccccccccctttattttctgccaaaacccccccccccggggaaaaaacccagaaa
aaaaaaccccaaaagtcccccccaaaaaaaaaaacccccaaaaggtccttacccgggcccaaaatccccgggcggccctttttccccccttttaacaccc
aaaagggggcgcaaacttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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