XB-CLONE-4352008
Clone Name: IMAGE:7027239
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Gene:
tmem111
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784527 [+]
taattaatcccccccccccccggatttccgtttccgctggctgactggctgaggaacatggggagccccgagttgctgttggactccagtatccgcctgt
gggtcgtcttacccatcgtctgtatcaccttcctcaccggcctcctccgccaccatgttacccgcctgctgcagggggagaagcgccccgaactggagcc
cctcagcgatagccaggtgctgatccggagccgcattcttagggagaacgggaaatacatcccccgacagtcgttcctcatgaggaagttctatttcaat
gaccaagagacgggatttttcaagaaaaccaagaggaaagtgatccagcggaacccaatgaccgaccccactatgatgaccgacatgatgaaggggaacc
tcaccaacgttcttcccatgatcctgattggagggtggataaactgggctttctctggcttcctgaccactaaggttccgttccccttgacgctccgatt
caagcccatgttacagcgtggcatcgagctggagtcactggacgcatcatgggttagctccgcctcctggtatttcctcaatgtctttggcctgcgcagt
atgtacaccctgctcttggggcaagataacgccgccgaccaaagccggatcatgcangatcagatgacgggggccgccctctccgtgccgccggacaccc
aacaagcttttcaggctgaatgggaagccatggagatctaggaacatcactgggcgctggaagccatcgagggaagatct
3' EST: CF784526 [+]gttctactgagccccggcaagttccagatcctgagcgatcagatcttcctcgatggcctccagcgcccagtgatgttcctagatctccatggcttcccac
tcagccttgaaagccttgttggtgtccggcggcacggagagggcggcccccgtcatctgatcctgcatgatccggctttggtcggcggcgttatcttgcc
ccaagagcagggtgtacatactgcgcaggccaaagacattgaggaaataccaggaggcggagctaacccatgatgcgtccagtgactccagctcgatgcc
acgctgtaacatgggcttgaatcggagcgtcaaggggaacggaaccttagtggtcaggaagccagagaaagcccagtttatccaccctccaatcaggatc
atgggaagaacgttggtgaggttccccttcatcatgtcggtcatcatagtggggtcggtcattgggttccgctggatcactttcctcttggttttcttga
aaaatcccgtctcttggtcattgaaatagaacttcctcatgaggaacgactgtcgggggatgtatttcccgttctccctaagaatgcggctccggatcag
cacctggctatcgctgaggggctccagttcggggcgcttctccccctgcagcaagcgggtaacatggtggcggaggaagcccgtgaagnaaggtgataca
gacgatgggttaagacgaacccaaagcgggatactgggagtcccaacagcaacttcggggcctcccccatgtttcctcaggccaggtccacccagccgga
aaacggaaaattccgggggggggggggggggaattaatttaacctcccgagga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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