XB-CLONE-4352005
Clone Name: IMAGE:7027238
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Gene:
c3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784528 [+]
taattaatccccccccccccccattctccagtctgcagcatggggagcggacttctctccctgtctctcctgctcctggtctctgggtcctacgcccaac
cgcaatgctcgcttatcacacccaatttgctgcgggcggagagtgaggagaccatcattatagacgcccaagggcaaaatgctgcttttgaagttgagat
catcatccatgaattccctcagaagaaactcaatttagccagtgccaaagtcaccctgaatagtgacaacaagttccttgggacggcgacagtaaagatt
ccctctgcagatgtacccaaagataagcagaataagcagtatgtgtatgtcagcctgaaatccaatgtgtgtgccctggagaaagtcgtcatgctgtcgt
accacagcggctacatcttcctccagactgacaagactatctacaccccaggatctacagttttgtatcggatttactccatgaactacaagatgcagcc
tatttcaaaaacgctcatcatcgaatttgtgaccccagatggtgttattgtgaagagagattccatcctccaggacagtaaatctggtatcatctcactg
tcccacaaactgccggaacttttgagccttggtgtctggaccatctccgcaaagtatgaagacacccctcaacagaattacaccaccaactttgaggtga
aagaatacgtgctaccctcaatggaaatcatcctgaagccagacactaacttcttctatgctgatgccaacctcttttggagtggatatt
3' EST: CF784529 [+]ttttttttttttttgagagcttgttctctgcggttcaccccaattcgctcccactggccgctgtaatccaggtaccgcgtggcaatgacacttggagtcg
tcgagatcatattctgctcgccgcagccagctggcaccacaatcagatgattcatgttgttaccatcaatggcatcttccaccatctgactgattggggt
accttgtaaagtgatggtggtgtcaatatcggttcgcggaacaacatttttggggttcagtgctttgatttcttcttcttgcactccatctttcccttta
acgtctggttctaatattacggtttttacatcctgtgcaatgcgcattccttctggcacaactttcagctttttacggacaccatcgttagcgaaagaac
ctgccacggatgccttcacctcaatgtcatgctggccaagagtgagcggcacaatgaccattgggacagcggtggaggagagggctccaatccaaacctc
ctgccggtacttcttcttaggcgtggccagactgcaaaactctggattgtgggtcagctctacccgcaccttaattctgtcattcctgtagttgtagaga
atggcccggatctccacctgctcgttcctcacaacagaatatggaagcttcaggtcgataaagaaatccttcatcaccttaatctcatagggctgagcca
cacacagaccttttggtttcggacaaactcacagccaggaacctccccagtagtgatgggaatctttccaagaaaacattaaggggtcttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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