XB-CLONE-4351999
Clone Name: IMAGE:7027235
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Gene:
slc25a20
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784533
[+]
taattaatcccccccccccccgctttcactcagcggataccggccttccccctcaccgcactcaggatggccaagcaaccgcagcctattagtcccatga
agaacttctttgccggcgggttcggagggatctgcctggtgtttgccgggcacccgctggacactattaaggttcggattcaaacccaaccaaagccggt
gccggggatacctcccttatattctggaacctttgattgctttaaaaagactcttgtaaatgagggtctccgcggcttgtacaaaggcatggcagctccc
attatcggtgtaacacccatgtttgctgtttgctttttcggctttggccttggtaaaaagctccagcaaaagcaccctgaggacattctgacatacccac
aactctttgctgccgggatgctgtcgggagtatttactactgcgatcatggctccaggtgagcgaatcaagtgcctcttgcagatccaggctgcctctgg
agaagtcaaatatgctggccccatggattgtgccaagcagctgtacagagaggccggaattcggggaatatacaaagggacggtgctcacacttatgaga
gatgttccggccagtggaatgtacttcatgacctacgaatggctgaagaacatcctcacaccagagggacacagtgtaagtgagctgagtgttccngaaa
ttctctttgctggcggaatggcagggaatctttaactgggctgttgcatttcctcagacgtgctaaaatct
3' EST: CF784532
[+]tttttttttttttttggttagaaggctaaagttttactgctgctaaaatgcatgtataaaatgtatccgttagagcgacacagactgtcccaacaagtgg
tctgtgagccgattaagcgccccattggctacacagagcgctggtgtgaggaacagtaaaaccctgttacacatataattattatacagagtatttgata
cttttagccatatacattcatatactgaagaggcttatcttccccaaatgactggggcactagcatgtacaccaaaagctgggtagggctatggggtgtc
tcagtctcagatgtaagacttcggtgttaagagaacagcactaatttagcaccgctcactaataaggcaatttattgtgtatttcagtccatgtagtcat
ggtgctctctgagaggtacctagcagcgcagtcaccgacagccacatcttcacttctacacctaattctgggtttcacatgttcggtgccatccagttca
ggaatttcatggctacctcaaatccaagaaaacatgccgcattagccgggaatgcccgcagcataactgccgtgaagcctttgtatagggatcctatgcc
ttcctctcggatcaactctcgtaaaacgtccctgaatccatttggatacttccctgctggagctgtctggaagcgagattttagcacgtctggaggaatt
gcaacagcccagttaaagattcctgccattccgccagcaaagagaattttcggaacactcagctcacttacactgtgtccctctgggtgtgaggatgttc
ttcaacca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: