XB-CLONE-4351975
Clone Name: IMAGE:7027224
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Gene:
tubb4b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784549 [+]
taattaatctcccccccccgagccagcgctccaaggtaaccggttcagttcggagtttagtacagagcaaccaactacagtcgccatgagggaaatcgtg
catctgcaggccggccagtgcggcaaccagatcggagccaagttctgggaagtgatcagcgatgaacatggaattgacccaaccggtacctaccacgggg
acagcgacctgcagctggagagaataaacgtctattacaatgaggcaacgggtggcaagtacgtcccccgcgctgtgttggtcgatcttgaacccggaac
catggactccgtgcgctcagggccatttgggcagatctttaggcctgacaactttgtgtttggtcaaagcggtgctggaaacaactgggccaagggccac
tacacagagggagcagaactggtggactccagtgttggacgttgtgaggaaagaagcagaaagctgcgactgtctccagggcttccagctgaaccactct
ctggggggcggcaccggctcgggcatgggcaccctgctcatccacaaaatcaggggaagagtaccccgacagaaccatgaatactttcagtggggtgccc
tccccccaaagtatctgaccaagtattagagccgtataaaccccccccctgtccggggcatctgcttggggacaaaaccccccaccgaatacccaacggt
ataaaataaaccaaggcccctggtcgaaacacccgcgcttcccgaaacccgctataaaaacccttttaccccacaaaccataagggggaacacttgtaca
cacctgtgggaattactcggccccaaacaaaggtaatgttggggctcaaaacaccacccgtggcttgtgtgccgggttttcccccggg
3' EST: CF784548 [+]ttcgacgaagtagctgctgttcttgttctgcacgttcagcatctgctcatccacctccttcatggacatgcggcctcggaagacggcggccacggtcagg
tagcggccgtgacgggggtcgcaggcggccatcatgttcttggcgtcgaacatctgctgggtgagctctggcacggttaaggcgcggtactgctggctgc
ctctgctggtgaggggggcaaagccgggcatgaagaagtgcagacgggggaaaggcaccatgttgacggccagtttgcgcaggtcggcgttcagctgccc
agggaaccgcaggcaggtggttaccccactcatggtggctgagaccaagtggttcaggtccccgtaggtgggggtggtgagtttgagggttctgaagcag
atgtcgtacagggcctcgttatctatacagtaggtctcgtctgtgttctctaccagctgatgcaccgacagggtggcgttgtacggctctactactgtgt
cagatactttgggggagggcaccacgctgaaagtgttcatgattctgtcggggtactcttccctgattttgctgatgagcanggtgcccatgcccgagcc
ggtgccgccccccagggagtgggtcagctggaagccctggagacagtcgcagctttctgcttctttcctcacaacgtccaacactgagtccaccagttct
gctccctctgtgtagtggcccttggcccagttgtttccagcaccgctttgaccaaacacaaagttgtcaggcctaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: