XB-CLONE-4351966
Clone Name: IMAGE:7027221
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Gene:
arpc1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784555 [+]
taattaatccccccccccccgatctgtgttgactgctcagagtccacggacagcagccgtctttccctgtacctactaacaggagcaaaaatggcctacc
acagttttctactggagcccattacctgtcatgcttggaacaaagatgcaacccaaattgccttctgtccaaatagtcatgatgttcacatttacaagaa
agatggagataaatggaccaaaatccatgagctgaaggaacataatggacaagtcacaggtattgactgggctcctgacagcaatcgcattgtaacctgt
ggtacggatcgtaatgcctatgtgtggactctaagaaacaatgtgtggaaaccgacactggtcattctcaggattaaccgtgctgcacgttgtgtaaagt
ggtcacctaaggagaacaagtttgcagtgggcagtggatccaggctaatttccatctgctacttcgagcaggaaaatgattggtgggtgtgtaagcacat
caagaaaccaatacgctcaacggtcctgagtctggattggcatccaaacaatgttcttctggcagctgggtccagtgattttaaaagcaggatctttttc
acttatatcaaagaaatcgaaaaacgccctgcccctactccatgggggctccaaaatgcccttttgggaaagttgatggtttgaagtctaacaccaccct
gtggggtggggtgccaaaagtttcctgcctttttccccacaaactggaaaaaccgcaatgggccttggggctggagccccatggaatagcccaccaaatt
ttgccatttgggccggaaaagtccccccccacgcgagaggtattgtgattacctatcacccccccct
3' EST: CF784554 [+]tttttttttttttttggaacaaatacttaaaatttattttgatagtgtagaataaaaattatgtccagcatattctgttccataagtaagcacagataca
ttaagcaaaacaaaattaattgcggatataggctttggcttttaaaagctttggaaaattttgtctgctgcaagacaaccttttataaatgaaatatgaa
gcagcttggcaacagagaatccacccttcctgtttgatcacttaatcttcagatctttcattgcagattccaggatcttgacgtcccagatgcacatccc
tccatctaagccagtggtacagaacttggaacactttgccttcccaccactcagcacagatatttggctgatgctgttcttgtgtaaggagtccagggta
acgttgttggtatcagagctggccttcttgtccaggttttgaaatctctctcgtgcagtcatccctctctgggaactctgcttggggatatcaagttttc
ccccaaaactcaaagtcccctgagcttcaacataagagtaaagtactggatagcagtcatggccagcagccaccaggctgttctcagtgataaaggtcaa
acacaagagcggcaaaatgtctgtaatcgaggtggtgactctcatcttcttgggggcatcaccaatgcaaatcgggctatcagggctcacccaagccctg
cgggccccactattgggaaaagccaaaactttgccccccacccacagctggctgctaaaactcaaaaaatcaattcccccaaaaaggattttttggaaac
cccacaggaaataaagggggccgagggccgttttctttccaaaattcgctttgac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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