XB-CLONE-4351957
Clone Name: IMAGE:7027218
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Gene:
abcf3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784561 [+]
taattaatccccccccccccccctctccaagatttctgacagttatgaggtcgatgaaaagatccagaatgttatgttgctgaagaaggatcaatcatcg
acagtggatgtgaagaggctggagaaagcagaagcaaaattgaagtctaagcaagagaaacggtctgagcgagatttacaaaaatcaactggaaatgtgg
taatggaggaagcctctgccagtcaagctgcaagtaagaaggagaaccggatagaatctagtggaaaaaacaaatcatatgacataaggattgaaaactt
tgatgtgtcatttggagaaagagttttgttgactggagcagagctgcatttggccactggccgtcgatatggattggttgggcgaaatggtttgggtaag
actatcctgttaaaaatgcttgcagggcgcagtcttcgtgtcccgtcacacatcagcatcctacacgtggaacaggaagtggccggtgatgaaacacctg
cattacagattgttctggagtgtgacacacttcgggaaagacttttttcccggaagagaaggaacttaacctccagattagcgcatgccggggttgaagg
atcataaagtttgcgctctctcctgatatccacccctcacttgggaaaaaattgaaacctgaaaaaagtttcctgacaacaagccctctgtcaatctttt
gactgggttttgggaatcctattacccctctactgtcattccccccttatggacccacaggtatttttctccgcggagaggttgggtccgcatttggtcg
cgcttggcccttgtgggtattttgggggcccccttcttttcgacccctgtttctctgcaccaccccctctctgcttttgtctctgaattaccaacacccc
ccctcaatttatttgttc
3' EST: CF784560 [+]tttttttttttttttggggattccgctctttatttacaacatgttagtttcacatctctcaacactgtcaataacttaagggaagctgtgtgctctgtgt
ttggcaatagttccacaattcatttggctggttacaatgtctaatgatgaagtactgggtgtatccaatcctgctaactaacaactggcatcttttaaaa
atcaatagtaacctacagtttgctaaaaaattagaaggtttcaatggataaaatattcaattttatggccaatttaacaggacttacacattataaagca
gccatttgagtattgtgacaacatttgttaagccattagttgccacagcagaaaacaaagacagggaaatagctgagcttacattacctggagatctgtg
cataacaatagaaatgtatttaaaacagaaaatataataattacatagaaaggcaatgtgttgttaatgtaagggcagcttttatatgaaaaatgtgatt
ggtattcagattcatttcagtagcagggaccagatacacagagcactgtgattagcaaaggtagcagccccagcctttactgcataaaacagaatcactt
cttacaaacagctgtttttgttattccctctttaaagtctgaaaataaatacagtcccttatatctcacaagtaatggctcagatcaaacagctggagta
tctgagttcaatgtagcaacttgctccatttaacgttcagtcctctgctccccacattttaccatggcaaccctcatggcgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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