XB-CLONE-4351954
Clone Name: IMAGE:7027217
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Gene:
ppp2ca
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784563 [+]
taattaatcccccccccccccgaagaagcaaaggaactggctgaggaggaggtgtggagccataaaggagaggttcgtgacgtcacggcggccattatac
tgtacaatacactctgagaaagaaggagccgtagctgcaaaattacagcggactgtctgataacgttatttctttattacctctactagctctgtacctc
ttacgaggcgctttgaagccctctggactgtgtccgtttagatactgtcgcctgagatcccggcactcccctgggttccgtattttattcaactgacggt
ccccacccctgccagcagcggtcgacttgttggttttctgacgccattcattggctcttggtccctccgagggcatacccggtgactgcctccctaggcc
gccggtcttttccttagacagccatggatgaaaaggcgttcacgaaggagttggatcagtggatcgagcagctgaatgagtgcaagcagctatcggagaa
ccaggtcaagactctgtgcgagaaagcaaaagaaatcttaacaaaaaaatcaaatgtccaggaaagttcggttgcccagtcacagtttgttggagatgtt
acccggacaaatttcacgattcttatggaactgtttcaaaaattggagggcaactcccccccaatacaaatttattttgttttaaggtgagaccctatct
tttgacacaaaggataatttacttcttgtttgaaaccctgtaaacgccccccttggttcccacccttaggggtgtttccctttcgggggaacacccttat
caaaatccccctcccaggggaaattcccgtagaacgcttataattaat
3' EST: CF784562 [+]ttttttttcttttttcgcaagtttattatgggggaagggaaaaagaaaggatactgctgcactgtcctgcagcaaagtcttacagaagatctggttccgg
tacagatgtatttgttattaactacagaaaataaaacagtcgcagacttgaaaattgatgcggaagggtgaaacgaaaaaaaaatagtaagcgcatatga
aatttaaattaaattgtattccctgaaaataaacatatctgaagtcacaataaaggcagcagggcccttggtacgcattgattaaaaacgtccatgtatc
cccttcacaccagagaagacagcagaatgctgttgcctcccctgcagcacagaaggggttaatccgactgtttctatcttatctataatcatgccaggta
gagctatagctaactggagttattgtattcacgtgttgttaatgttttagtttttgcagtctctccgagaaagcaaatgtactacaagtagtgctatgcc
aggaaacagattttttttttttttctctttgacagacaatgccacttcagcatgcaaggacagggtctctaaggtggtcacgggctgttgaaacaagagg
cttttatggcacatcttttggtccatcatttaagagtacataagtgacaacgctcccctgaatttgaagttactgctgcttttccgagatctcatgacaa
aatcctgctggtagcaatactgtacaagaagtaaaattctgtttacaggaaagtattcngggtgtgcgaacggttcacatgtgggctcctcccctgcgtg
ggtgctggggccaaactgcaaagaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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