XB-CLONE-4351949
Clone Name: IMAGE:7027215
|
Gene:
apoa1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC077663 [+]
ACAGGAGCAACTAACCAGCAAAGCAGGTTGGAGAGTTTGCAACTATGAAGAACCTAGTCCTACTAGCAGTACTGTTCCTCTCAGGAACACAGGCAAAGTA
TTTCCCTTGGCAGCATGAAGAGCCCCACACTCCAGCCCAGCAGGTGCGGGAAGTCTTAGAGAGCTACGTACACAAAGTTCGGGACTTGGGCAGAGAAGCT
GTGAGCCAACTGGAGACCTCTGATCTCGGAAAACAACTTGACCTGAAGATCACTGAGAAGCTTGACACCTTTGGTACCAATGCTATGAACCTGAGAAAGC
AAATAAATCCCTATGTAGAAAATATCAGGGAACAGGTGTCCAAAGAGCTAGAAAAGGAACTCCCAGTAATCAAGGAGAAAATCCGCCCCATCCTGGAAGC
TTTCCAGAAACGTTGGGCTGATGATGCAAAGGCTTACCAGGAGAAAATCTCTCCCCTGGTAGCTGAGCTTCAAAAGCAAACCAAGAACAACCTGGATTCC
TTGTATAAAAAGATCACTCCTATTGCTGAAGAGATAAGGGACAAAGTGAGAGTGGATGTTGACTCCCTTCGCTCAAGGCTCACACCCTACAGTGAAGAGG
TCAGGGTAAAGCTGGTCGAGAAACTAGAAGAGCTTAAGGCCAATGCAGGTCCCAGAGCTGAGGAGTACAGAGCCCAACTGTCACAGCATATTGAGAATTT
GAAGGAGAAGTATGGACCCCTGGCCCAGAGCATCAGAGAACGCCTGGTACCCCATGCAGAGGAGGCCAGAAACAACCTGGCAAAAATTTGGGAAAATCTG
AGAGCCAAGCTCCAAAGACAGGGTGCCTAAAACCTTCACCTTCTATCTGTGAAGATGTATGTTGGTGCTTCAAACCTCATCCAAACTTCTCGCTCACACA
ACCACCTATTATCTCAACTTATACTATTACCATGATGCTGGCCTGAGAGAGGACATGTTTACATGCTGCTAGCCCTTACATTATCTCCAAGAGGGCAACC
AATATTGATATGAAATGAATTGCTACATATCTTTTATTGTTTACATATTTCATATAAATTGCCCTGAGGGCACATGGTCCTCTCTCATGTTTCAATGTGA
AAATAAAGACATTAGTATATAAAAAAAAAAAAAAAA
5' EST: CF784566 [+]taattaatcccccccccccccacaggagcaactaaccagcaaagcaggttggagagtttgcaactatgaagaacctagtcctactagcagtactgttcct
ctcaggaacacaggcaaagtatttcccttggcagcatgaagagccccacactccagcccagcaggtgcgggaagtcttagagagctacgtacacaaagtt
cgggacttgggcagagaagctgtgagccaactggagacctctgatctcggaaaacaacttgacctgaagatcactgagaagcttgacacctttggtacca
atgctatgaacctgagaaagcaaataaatccctatgtagaaaatatcagggaacaggtgtccaaagagctagaaaaggaactcccagtaatcaaggagaa
aatccgccccatcctggaagctttccagaaacgttgggctgatgatgcaaaggcttaccaggagaaaatctctcccctggtagctgagcttcaaaagcaa
accaagaacaacctggattccttgtataaaaagatcactcctattgctgaagagataagggacaaagtgagagtggatgttgactcccttcgctcaaggc
tcacaccctacagtgaagaggtcagggtaaagctggtcgagaaactagaagagcttaaggccaatgcaggtcccagagctgaggagtacagagcccaact
gtcacagcatattgagaatttgaaagaagagtatggacccctggcccaaagcatcagagaacgcctgggtaccccatgcanagggaggcccagaaacaaa
cctgggcaaaaattttggggaaaaaa
3' EST: CF784565 [+]tttttttttttttttatatactaatgtctttattttcacattgaaacatgagagaggaccatgtgccctcagggcaatttatatgaaatatgtaaacaat
aaaagatatgtagcaattcatttcatatcaatattggttgccctcttggagataatgtaagggctagcagcatgtaaacatgtcctctctcaggccagca
tcatggtaatagtataagttgagataataggtggttgtgtgagcgagaagtttggatgaggtttgaagcaccaacatacatcttcacagatagaaggtga
aggttttaggcaccctgtctttggagcttggctctcagattttcccaaatttttgccaggttgtttctggcctcctctgcatggggtaccaggcgttctc
tgatgctctgggccaggggtccatacttctccttcaaattctcaatatgctgtgacagttgggctctgtactcctcagctctgggacctgcattggcctt
aagctcttctagtttctcgaccagctttaccctgacctcttcactgtagggtgtgagccttgagcgaagggagtcaacatccactctcactttgtccctt
atctcttcagcaataggagtgatctttttatacaaggaatccaggttgttcttggtttgcttttgaagctcagctaccangggagagattttctcctggt
aagcctttgcatcatcagcccaacgtttctggaaagcttccaggatggggcggattttctcccttgatactgggagttcccttttctagctctttggaca
cctggtcccctgatattttctacataggggatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: