XB-CLONE-4351946
Clone Name: IMAGE:7027214
|
Gene:
foxd1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC081361 [+]
AAGCTTGTGTAGCGCTGCCCGACACTCCTTGCAGCTCCCTCTCCGCCTGTGCGGGCCAATGACCGGCCGCTATAGCCGGGGAGCCGAGACCCATCGCCCG
GAGCCCGGGGTTGCCCCTATTGCCCCTGTGTGTGTGCCCGATGCAGTCGGCCCCCCAGCAGCCTTATGATTTGCCTCCCAAGCCATGACTCTGAGCTCTG
ACATGTCCGATGTTCTTGCTGAAGAGACCGACATAGACGTGGTGGGAGAAGACGACGAGCCGCGGGCAGAGGAGGAAGAGGACGAGGATCTCCATGGGGA
CCTCCTGCCCACATCCCCGCAGAGCTCGGCCACCAAAGACCCCTACAAGGGGACGGGCGGCGGGGGCGGCAGGAGCGCCCTGGTGAAGCCCCCCTACTCG
TACATCGCGCTGATCACTATGGCGATACTGCAGAGCCCCAAGAAGCGCCTGACCCTCAGCGAGATCTGCGAGTTCATCAGCAACCGCTTCCCCTACTACC
GGGAGAAGTTCCCCGCCTGGCAGAACTCCATCAGGCACAACCTGTCCCTCAACGACTGCTTCGTCAAGATCCCCCGGGAGCCCGGCAACCCCGGCAAGGG
CAACTACTGGACGCTGGACCCGGAGTCCGCTGACATGTTTGACAATGGGAGTTTTCTCAGGAGGAGGAAGCGCTTTAAGAGACAACAAGCGCCGGAGCTG
GTGCTTAGGGAGCCCGGCCACTTCCTTCCAGCCTCTGCCTACAGCTACGGACCCTACAGCTGCGCCTATGGCATCCAGCTACAGCCTTTCCACCCACACT
CAGCCTTGATTGCCTTCCAGCAGCAGCAGCAGCAAGCCAGGCAGCAGCCCCCCAGCCTCCCCCCCATGGCGGCCCCAGCCCTTATGCCACCTGCTGCCCA
GGATCTATCAAGGACCTGCACTTTCTACCCTCACCAGCTCAGCCCAGCAGCCCTGCCACCTTCCCTGCAGAGCAAGTCCAGCTCAGCCCTGGCTAGATCC
ACCTTTTCTATTGAGAGTATCATAGGTGGGGACCTCAACCCTGGCCAGTGTAACTCCCCCAAAGCAGCTGGGGTCCCTGTGATCTCTAGGGCCCTGGTGA
CTTTCTCCAGCTCACAGGCTGCTGCAGCTTTAGGGGGGACCCTGCAGCCTGGGACGGTGCTTACAAACCACTAGGATTTCTAATTCTTAAATCAGACATT
TCTGAGAAGGTAGGAGGCTTTTTATGGATTATGCATCATGTTTTGCTTTGGGGGTTTTTTCCAGGGATGCCCAACCTACTTCCTTCCTTCTGGTGTTAAA
CTACAAGTCTCAGGCATGGCTGCCGTAGCATGCTGGGAACTGTAGTTCTAACACAAGCAGAGGGAGGCAGGCTGGAGAGCTGTGTTCCAGCAAGGAATGC
AAAATATTTCAGCCTGAACTCTATGCCAAACCAAGGTTCCCTGCCAACGGATTGCAGCGCCTTTACTTGCCCTTTAGAGATCCCTGTCTCTTCTATTTGT
ACAGTTTTACTGTGACTCAGGTCTGAAGATTCCCAGCATTTGTGTAGGAAGCAGAAATGCAGCAATATCTTGTAGCTCACACACACACCAAGTGCTTTTG
TGGCGAAATGATTTCCATGTTTACACAGAGACAATCTTCCCCATTTCAATTTTAGCCTGTGTTTTGTATCATAATAGGGGGTTGTGTTTTTGTACGAAAG
TATTTCTTATTTACAAGAATTAATGTAACGCATACTGCCAAGTATCATTGTACCTAGGCAATGTACGATTTTTTTTCACATTTACAAAAAAAAAAATAAA
AATTAAATAAAAACAAAATGTTTAAAAAAAAAAAAAAAA
5' EST: CF784568 [+]taattaatcccccccccccccaagcttgtgtagcgctgcccgacactccttgcagctccctctccgcctgtgcgggccaatgaccggccgctatagccgg
ggagccgagacccatcgcccggagcccggggttgcccctattgcccctgtgtgtgtgcccgatgcagtcggccccccagcagccttatgatttgcctccc
aagccatgactctgagctctgacatgtccgatgttcttgctgaagagaccgacatagacgtggtgggagaagacgacgagccgcgggcagaggaggaaga
ggacgaggatctccatggggacctcctgcccacatccccgcagagctcggccaccaaagacccctacaaggggacgggcggcgggggcggcatgaccgcc
ctggtgaagcccccctactcatacatcgcgctgatcactatggcgatactgcacagccccaagaagcgcctgaccctcagcgagatctgcgagttcatca
gcaaccgcttcccctactaccgggagaagttccccgcctggcataactccatcatgcacaatctgtccctcaacgactgcttcgccaagatcccccggga
gtccgggcatcccccgccagggccaactattggacgcctgggacccggagtccgcttacatggttcgaaaatggggattttttcctcaggaaggaaggaa
agccgcttttaaagagaacatactaagccgcccgcggagcttgtggccccctccaggggaattcccggggcactacctttcccgtttaccgttgcttctt
ggcctcttaacgagtctcaccctggtaacctccccaacaagatctttgttcgtcccctccgttgtggcgctatatcttaagcgct
3' EST: CF784567 [+]tttttttttttttttaaacattttgtttttatttaatttttatttttttttttgtaaatgtgaaaaaaaatcgtacattgcctaggtacaatgatacttg
gcagtatgcgttacattaattcttgtaaataagaaatactttcgtacaaaaacacaaccccctattatgatacaaaacacaggctaaaattgaaatgggg
aagattgtctctgtgtaaacatggaaatcatttcgccacaaaagcacttggtgtgtgtgtgagctacaagatattgctgcatttctgcttcctacacaaa
tgctgggaatcttcagacctgagtcacagtaaaactgtacaaatagaagagacagggatctctaaagggcaagtaaaggcgctgcaatccgttggcaggg
aaccttggtttggcatagagttcaggctgaaatattttgcattccttgctggaacacagctctccagcctgcctccctctgcttgtgttagaactacagt
tcccagcatgctacggcagccatgcctgagacttgtagtttaacaccagaaggaaggaagtaggttgggcatccctggaaaaaacccccaaagcaaaaca
tgatgcataatccataaaaagcctcctaccttctcagaaatgtctgatttaagaattagaaatcctagtggtttgtaagcaccgtcccagctgcaagttc
ccccctaaaactgcagcaacctgtgagctggagaaagtcacccgggcccctagagattacaggggaccccgcctgcttttgggggagttacactgggcca
gggtttgaggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: