XB-CLONE-4351936
Clone Name: IMAGE:7027210
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Gene:
pcbd1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082730 [+]
GAGCAACAGCTGAGCTGATTGTGCTGCGGAGACTGAGACTGAGACTGCAGATCCACTAGTTGCTTTAGGAGACATGGCTGGAAAAGCACACAGACTCAGC
GGGGAGGAAAGAGAGCAACTGCTGCCTAACTTGAGAGCAGTAGGCTGGCATGAACTGGATGGCAGAGATGCAATATGCAAAGAATTTCATTTTAAAGACT
TCAACCGAGCCTTTGGATTCATGACAAGGGTGGCTCTGCAGGCTGAAAAACTTGATCACCATCCAGAGTGGTTCAATGTCTATGATAAGGTTCACATAAC
ATTGAGCACCCATGATTGCGGTGGTCTTTCTGAGCGAGACATCAACTTGGCTAGTTTTATTGAACAAATAGCTGCCAGCCTGTCTTAGGAAACACATTCC
CAATTGTCATGTAGTTTCACAATGTCAGTTTCCAATGTTTCCCCTTGGATTTGTTTTATATACATATTGATTTAATGCCCAGTGATATTCTTTAGACTCT
GTATAACATTCCATACTTGGGTGTTGTTCACAATGACAACTTGAAGCAAAACTTTTTAATGTTTTTTTTTTCATGTTTTTTTTTCTCCTTAATTTTAGTA
ACTATAATAAGTAAGGCAAATCTCTAAAAGGATATTGTGATTTTAGTATTAAATGCATTTGGTCACTTCAGGCTATACCAATGCAGCAATACAGTAGCTG
ATAAGGGAAACCCCCTTGGATGTCGTTGCTGTTGCTTCTGGAGGGTCCTCTGTGATAAGCCACAACTGACCGGTCAACATAATCATTTGTGCTTCTCTGG
ACAACTTGCAGAGTGACCCCCCTCATTTTCAATTTTTTTATCCCTTTTTGATTTTGTTAATACTGCTGTTCTGTTAATCCTGTCTGTAGTGAGAATGTGT
ATAATCTTATGCAATAGCCTTACTTTTAAACAGTGTTATGGTTAAGGCCTCAGTTAATGTTTCAGAAGAGTCTGTTACCATTCAAGTGTTTTGTGTATTT
GTTCATTACACTTGTTTCTGTTATTGTATCAATTTCATGCAAATAATTACTTTTACCTAATAAAAAGACCTAGACTACCTAACAAACAATGGTACTAGGA
CACAATATAAAAAAAAAAAAAAAA
5' EST: CF784574 [+]taattaatcccccccccccccgagcaacagctgagctgattgtgctgcggagactgagactgagactgcagatccactagttgctttaggagacatggct
ggaaaagcacacagactcagcggggaggaaagagagcaactgctgcctaacttgagagcagtaggctggcatgaactggatggcagagatgcaatatgca
aagaatttcattttaaagacttcaaccgagcctttggattcatgacaagggtggctctgcaggctgaaaaacttgatcaccatccagagtggttcaatgt
ctatgataaggttcacataacattgagcacccatgattgcggtggtctttctgagcgagacatcaacttggctagttttattgaacaaatagctgccagc
ctgtcttaggaaacacattcccaattgtcatgtagtttcacaatgtcagtttccaatgtttccccttggatttgttttatatacatattgatttaatgcc
cagtgatattctttagactctgtataacattccatacttgggtgttgatcacaatgacaacttgaagcaaaagcttttaatgtattttttttcatgtatt
tgttttccccttagttttaggtactactataattcaggacaatcctctaaaaggatattggtgatttctaggaataaaaatgccatttgggcccctttca
ggcttaagccctactgcgcaccccctacagacacccccggaatttgggggaaaaccccccctttgggattggccccctggcctggtttggatattcttgg
ggagggcggcccgcctctgtgacactccgacccctactatattggaacccgggggcccaaaaatatctcgac
3' EST: CF784573 [+]tttttttttttttttatattgtgtcctagtaccattgtttgttaggtagtctaggtctttttattaggtaaaagtaattatttgcatgaaattgatacaa
taacagaaacaagtgtaatgaacaaatacacaaaacacttgaatggtaacagactcttctgaaacattaactgaggccttaaccataacactgtttaaaa
gtaaggctattgcataagattatacacattctcactacagacaggattaacagaacagcagtattaacaaaatcaaaaagggataaaaaaattgaaaatg
aggggggtcactctgcaagttgtccagagaagcacaaatgattatgttgaccggtcagttgtggcttatcacagaggaccctccagaagcaacagcaacg
acatccaagggggtttcccttatcagctactgtattgctgcattggtatagcctgaagtgaccaaatgcatttaatactaaaatcacaatatccttttag
agatttgccttacttattatagttactaaaattaaggagaaaaaaaaacatgaaaaaaaaaacattaaaaagttttgcttcaagttgtcattgtgaacaa
cacccaagtatggaatgttatacagagtctaaagaatatcactgggcattaaaatcatatgtatataaaacaaatcccaggggaaacattggaaactgac
attgtgaaactacatgacaattggggaatgttgtttccctaaaacaggctggcacctattttgttcaataaaacctacccaagttgaaggcctccctca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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